致密层骨软骨复合支架的制备及其修复关节骨软骨缺损

发布时间：2018-06-10 16:44

本文选题：磷酸钙类 + 膝关节　；参考：《中国组织工程研究》2017年02期

【摘要】：背景:理想的骨软骨组织工程支架应巧妙地模拟人体正常关节骨软骨结构。目的:基于关节骨软骨的解剖结构、生理功能,制备致密层骨软骨复合支架,同时观察其修复兔关节骨软骨缺损的效果。方法:利用快速成型技术在三维立体包芯结构骨架表面喷涂乳酸-羟基乙酸共聚物/β-磷酸三钙有机溶液,形成0.5 mm的致密层;利用"溶解-粘连"工艺能将定向微管结构软骨支架与致密层相互连接,形成致密层骨软骨复合支架。取60只家兔,制备左膝关节软骨全层缺损,随机分3组,实验组植入致密层骨软骨复合支架,对照组植入定向微管结构软骨支架,空白对照组不植入任何材料,修复后12,24周进行缺损部位大体与组织学观察。结果与结论:1大体观察结果:对照组修复后12周存在明显缺损部位,未见明显修复痕迹;24周缺损面积减少,可见新生组织覆盖,但表面粗糙。实验组修复12周后缺损部位表面平整,质地较软,与周围组织边界不清;修复后24周,被透明软骨样组织覆盖,表面平整。空白对照组修复效果较差;2组织学观察结果:修复后12周,对照组缺损部位出现形态不规则的骨痂,但未形成骨小梁;实验组出现新生骨,软骨厚度与正常软骨接近,并且软骨下存在不规则骨小梁。修复后24周,对照组出现新生软骨,但厚度不均,高低不平,骨小梁结构不规则;实验组组织与正常组织无明显差异,软骨表面光滑;空白对照组修复效果较差;3结果表明:致密层骨软骨复合支架接近人体正常关节骨软骨结构,可促进关节软骨缺损的修复。
[Abstract]:Background: the ideal scaffold for osteochondral tissue engineering should mimic the normal articular cartilage structure. Aim: based on the anatomical structure and physiological function of articular cartilage, the composite scaffolds of dense lamellar cartilage were prepared and the effect of repairing articular cartilage defects in rabbits was observed at the same time. Methods: rapid prototyping technique was used to spray lactic acid-glycolic acid copolymer / 尾 -tricalcium phosphate organic solution on the surface of three-dimensional core structure skeleton to form a dense layer of 0.5 mm. The composite scaffold of dense layer bone cartilage can be formed by connecting the oriented microtubule cartilage scaffold with the dense layer by the "dissolving-adhesion" process. Sixty rabbits were randomly divided into 3 groups: the experimental group was implanted with compact lamellar cartilage composite scaffold, the control group was implanted with directional microtubule cartilage scaffold, and the blank control group was not implanted with any material. Gross and histological observations of the defect were made 24 weeks after repair. Results and conclusion: in the control group, there were obvious defects at 12 weeks after repair, and no obvious signs of restoration were found in the control group. The defect area was decreased after 24 weeks, but the surface was rough. After 12 weeks of repair, the defect in the experimental group was smooth and soft, and the boundary with the surrounding tissue was not clear. 24 weeks after repair, the defect was covered with transparent cartilage tissue and the surface was smooth. The results of histological observation showed that the defect site of control group had irregular shape callus but no trabecular bone at 12 weeks after repair, and the new bone appeared in experimental group, and the thickness of cartilage was close to that of normal cartilage. And there are irregular trabeculae under cartilage. 24 weeks after repair, new cartilage appeared in the control group, but the thickness was uneven, bone trabecular structure was irregular, there was no significant difference between the experimental group and normal tissue, and the cartilage surface was smooth. The results of the blank control group showed that the compact lamellar cartilage composite scaffold was close to the normal articular cartilage structure and could promote the repair of articular cartilage defect.
【作者单位】：郑州大学附属洛阳中心医院;河南大学第一临床学院;
【基金】：2016年洛阳市科技计划医疗卫生项目(1603002A-15) 河南省重点实验室研究项目(J01501153)~~
【分类号】：R318.08;R684

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