基于近红外光谱分析的脊髓损伤后神经细胞活性检测方法

发布时间：2018-06-11 19:08

本文选题：脊髓 + 神经递质　；参考：《光谱学与光谱分析》2017年02期

【摘要】：交通意外、高空跌落、机械撞击等事故,可能导致人体脊髓损伤,从而中断了人体神经信号的传输信道,致使部分肢体功能的丧失。由于目前没有脊髓受损部位神经细胞活性检测的方法,而常规的检测仪器如X射线、CT等传统仪器不能提供神经细胞活性的任何信息,医生无法对患者病情有更清晰的了解,这就可能延误最佳的治疗时机,致使患者终生瘫痪。光谱学方法可以检测细胞组织的改变,且近红外光谱技术是一种测量速度快,操作简便的无损测量技术。所以,针对现有医学检测设备的不足,在动物实验的基础上,根据物质在近红外光波段的吸光特性,并结合近红外光谱分析技术的定性判别和定量分析的特性,通过对脊髓损伤后神经细胞活性的检测,采用聚类算法对脊髓损伤部位的神经特异性核蛋白和神经递质进行分类和仿真,并采用偏最小二乘算法计算其含量,实现了脊髓损伤部位神经细胞活性的精确检测。该方法为脊髓损伤后神经细胞活性的检测提供理论依据,给患者带来肢体功能重建和康复的希望,为临床医学提供了一种无创检测神经细胞活性的方法。
[Abstract]:Accidents such as traffic accidents, high altitude falls and mechanical impacts may lead to spinal cord injury, thus interrupting the transmission channel of human neural signals, resulting in the loss of some limb functions. Since there is currently no method for the detection of nerve cell activity in the injured spinal cord, and conventional instruments such as X-ray scanning and CT cannot provide any information on the activity of nerve cells, doctors cannot have a clearer understanding of the patient's condition. This could delay the best treatment and lead to life-long paralysis. The near infrared spectroscopy (NIR) is a fast and easy nondestructive technique to detect the change of cell tissue. Therefore, in view of the deficiency of the existing medical detection equipment, on the basis of animal experiments, according to the absorption characteristics of matter in near-infrared band, and combining with the qualitative discrimination and quantitative analysis characteristics of near-infrared spectroscopy, Through the detection of nerve cell activity after spinal cord injury, the neuronal specific nuclear protein and neurotransmitter of spinal cord injury were classified and simulated by clustering algorithm, and the content of neuronal specific nuclear protein and neurotransmitter were calculated by partial least square algorithm. The accurate detection of nerve cell activity in spinal cord injury was realized. This method provides a theoretical basis for the detection of nerve cell activity after spinal cord injury, brings hope to patients with limb function reconstruction and rehabilitation, and provides a non-invasive method for the detection of nerve cell activity in clinical medicine.
【作者单位】：西安邮电大学通信与信息工程学院;西安邮电大学校医院;西北工业大学电子信息学院通信工程系;西安电子科技大学通信工程学院;
【基金】：国家自然科学基金项目(61172071,61201194) 陕西省自然科学基金基础研究计划项目(2014JQ8318) 陕西省国际科技合作与交流计划项目(2015KW-013)资助
【分类号】：R651.2;O657.33

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