NO舒张犬肺血管的主要作用通路

发布时间：2018-06-12 12:47

本文选题：缺氧性肺动脉高压 + 一氧化氮　；参考：《南华大学》2015年硕士论文

【摘要】：目的:在犬缺氧性肺血管收缩模型的基础上研究一氧化氮是否直接作用于肺小动脉降低肺动脉压,从而进一步探讨其主要作用通路。方法:选择健康的雄性成年比格犬16只,随机分为2组:正常组8只,切断单侧迷走神经组8只(其中切断左或右侧迷走神经各4只)。动物体、肺双循环建立成功后,在缺氧性肺血管收缩模型的基础上通过呼吸道或肺循环的氧合器给予恒定浓度的一氧化氮,持续监测平均肺动脉压及平均动脉压的变化,在每个处理结束前从左(右)心房采取血标本。切断单侧迷走神经组饲养一个月,分别阻断进入左/右肺的动脉进行上述实验。结果:缺氧导致肺动脉压较基础值明显升高(P0.01),经呼吸道吸入一氧化氮后,肺动脉压恢复正常(与缺氧比较P0.01);而经肺循环氧合器吸入一氧化氮后与缺氧相比较,肺动脉压无明显变化,两种处理无统计学差异(P0.05)。切断单侧迷走神经后,有迷走神经侧或无迷走神经侧肺,缺氧或吸入一氧化氮均不能导致肺动脉压变化(P0.05)。结论:一氧化氮降低肺动脉压的作用是通过迷走神经途径而不是直接作用于肺小动脉。
[Abstract]:Aim: to investigate whether nitric oxide acts directly on pulmonary arterioles to reduce pulmonary artery pressure and to further explore its main pathway based on the hypoxic pulmonary vasoconstriction model in dogs. Methods: sixteen male adult Beagle dogs were randomly divided into two groups: normal group (n = 8) and unilateral vagotomy group (n = 8). On the basis of hypoxic pulmonary vasoconstriction model, the animal and pulmonary double circulation were given a constant concentration of nitric oxide through the oxygenator of respiratory tract or pulmonary circulation, and the changes of mean pulmonary artery pressure and mean arterial pressure were continuously monitored. Blood samples were taken from the left (right) atrium before each treatment. The unilateral vagotomy group was fed for one month and the artery entering the left / right lung was blocked. Results: compared with hypoxia, pulmonary artery pressure increased significantly (P 0.01). Pulmonary artery pressure returned to normal after inhaling nitric oxide in respiratory tract (compared with hypoxia, P 0.01), but after inhaling nitric oxide through pulmonary circulatory oxygenator, it was compared with hypoxia. There was no significant change in pulmonary artery pressure and there was no statistical difference between the two treatments (P 0.05). After unilateral vagus nerve transection, there were vagus nerve side or no vagus side lung, hypoxia or inhalation of nitric oxide could not cause pulmonary artery pressure change (P 0.05). Conclusion: the effect of nitric oxide on pulmonary artery pressure is via vagus pathway rather than in pulmonary arteriole.
【学位授予单位】：南华大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R614

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