脂肪间充质干细胞复合利福平缓释微球在脊柱结核动物模型中的应用

发布时间：2018-06-12 14:40

本文选题：迟效制剂 + 干细胞　；参考：《中国组织工程研究》2017年26期

【摘要】：背景:制备一种可直接植入骨结核病灶内且在骨结核周围组织能够长期保持一定抗结核药物浓度,增强骨缺损修复的新型生物材料,成为骨结核临床研究的热点。目的:制备脂肪间充质干细胞与利福平缓释微球复合物,初步探讨其对兔脊柱结核模型的治疗作用。方法:合成利福平-壳聚糖-海藻酸钠缓释微球,将其与脂肪间充质干细胞复合培养,制作抗结核性骨组织工程复合体。取40只取新西兰大白兔,制作L6椎体结核模型,造模成功后随机分4组干预,对照组不进行任何干预;利福平组每天灌胃给予利福平;干细胞组在椎旁注射脂肪间充质干细胞,每天灌胃给予利福平;实验组在椎旁植入抗结核性骨组织工程复合体,每天灌胃给予利福平。给药8周后,通过X射线、CT初步观察病灶情况。结果与结论:对照组L5、L6椎体都有明显骨质破坏,形成炎性肉芽组织,椎间隙变窄,2只出现明显后凸畸形,5只兔腰大肌肿胀,腰大肌内可见低密度暗区。利福平组兔模型中5只兔L5和L6椎体有中度骨质破坏,2只兔腰大肌肿胀。干细胞组兔模型中2只兔L5和L6椎体有中度骨质破坏,3只兔L6椎体上部有轻度骨质破坏,3只兔腰大肌肿胀。实验组中4只兔L6椎体上部有轻度骨质破坏,L5、L6椎间隙无明显改变,无腰大肌肿胀发生。结果表明,脂肪间充质干细胞与利福平缓释微球复合物可抑制结核杆菌生长,减少椎体骨质破坏,对动物脊柱结核模型具有一定的治疗作用。
[Abstract]:Background: the preparation of a new biomaterial which can be implanted directly into the focus of bone tuberculosis and can maintain a certain concentration of antituberculous drugs in the tissue around bone tuberculosis for a long time and enhance the repair of bone defect has become the focus of clinical research on bone tuberculosis. Aim: to prepare adipose mesenchymal stem cells and rifampicin sustained-release microspheres for the treatment of spinal tuberculosis in rabbits. Methods: rifampicin-chitosan sodium alginate sustained-release microspheres were synthesized and co-cultured with adipose mesenchymal stem cells to prepare anti-tuberculous bone tissue engineering complex. Forty New Zealand white rabbits were used to make L6 vertebral tuberculosis model. After the model was successfully established, the rats were randomly divided into 4 groups, the control group did not intervene, rifampicin group was given rifampicin daily, and the stem cell group was injected with adipose mesenchymal stem cells by paravertebral injection. Rifampicin was given daily by gavage, and rifampicin was given daily by intragastric administration of rifampicin in the experimental group after implantation of antituberculous tissue engineering complex. After 8 weeks of administration, the lesions were preliminarily observed by X-ray CT. Results and conclusion: in the control group, the vertebrae of L5 and L6 had obvious bone destruction and inflammatory granulation, and 2 rabbits had obvious kyphosis deformities, and 5 rabbits showed swelling of the psoas major muscle, and the low density dark area could be seen in the psoas major muscle. In rifampicin group, 5 rabbits with L5 and L6 vertebrae had moderate bone destruction and 2 rabbits had swelling of psoas major muscle. In the stem cell group, there were moderate bone destruction in L5 and L6 vertebrae in 2 rabbits and mild bone destruction in the upper part of L6 vertebra in 3 rabbits, and swelling of psoas major muscle in 3 rabbits. In the experimental group, there was no obvious change in the intervertebral space of L5 and L6, and no swelling of the psoas major muscle in the upper part of the L6 vertebra of 4 rabbits. The results showed that the compound of adipose mesenchymal stem cells and rifampicin sustained-release microspheres could inhibit the growth of Mycobacterium tuberculosis and reduce the destruction of vertebral bone.
【作者单位】：武汉市医疗救治中心结核科;
【基金】：国家自然科学基金(30973087) 湖北省自然科学基金(2013CFB513)~~
【分类号】：R318.08;R-332;R529.2

【参考文献】