脊髓挫裂伤致大鼠认知功能受损的实验研究

发布时间：2018-06-12 18:52

本文选题：脊髓损伤 + 认知障碍　；参考：《第三军医大学学报》2017年01期

【摘要】：目的探究脊髓损伤后的认知、情绪改变以及脑内相关区域病理变化和内质网应激相关分子表达情况。方法 120只成年SD雌性大鼠分为损伤组和对照组(每组60只)。制备脊髓T10节段的Allen法打击损伤模型(打击力度20 g×25 mm),对照组只接受椎板切除术。BBB评分观察损伤后运动功能恢复情况,Morris水迷宫和高架十字迷宫分别检测大鼠学习记忆功能和情绪改变。尼氏染色观察大脑神经细胞形态数量,快速高尔基体染色检测海马区域树突棘密度。Western blot检测GRP78和Caspase-12蛋白在海马区域的表达变化。结果高架十字迷宫检测损伤大鼠进入开臂次数明显减少,水迷宫空间探索测试结果显示损伤动物跨越平台次数明显下降(P0.05,P0.01)。尼氏染色和快速高尔基体染色结果显示大鼠皮质和海马区域神经元形态和数量异常,海马区域树突棘密度下降(P0.05,P0.01)。Western blot检测结果显示脊髓损伤后内质网应激相关蛋白GRP78表达上调(P0.05,P0.01)。结论脊髓损伤后成年大鼠产生持续学习记忆能力下降,同时出现焦虑的情绪变化。这可能与脊髓损伤后海马和皮质区域发生的内质网应激引起海马与皮质区域神经细胞受损有关。
[Abstract]:Objective to investigate the changes of cognition, emotion, pathological changes and ER stress related molecules in brain after spinal cord injury (sci). Methods 120 adult SD female rats were divided into injury group and control group (60 rats in each group). Establishment of spinal cord T10 injury model with Allen method (20 g 脳 25 mm). The control group only received laminectomy. BBB score was used to observe the recovery of motor function after injury. Morris water maze and elevated cross maze were used to detect the learning of rats. Memory function and emotional changes. The morphological number of neural cells was observed by Nissl's staining and the expression of GRP78 and Caspase-12 proteins in the hippocampus was detected by rapid Golgi body staining and the density of dendritic spine in the hippocampal region. Western blot was used to detect the expression of GRP78 and Caspase-12 protein in the hippocampus. Results the number of entry into the open arm of the injured rats was significantly reduced by elevated cross maze test. The results of space exploration of water maze showed that the number of cross platform crossing of the injured animals was significantly decreased (P 0.05) and P 0.01 (P 0.01). The results of Nissl staining and rapid Golgi body staining showed that the morphology and number of neurons in cortex and hippocampus of rats were abnormal, and the density of dendritic spine in hippocampus was decreased. Western blot analysis showed that the expression of ERS-associated protein GRP78 up-regulated P0.05 and P0.01a after spinal cord injury. Conclusion after spinal cord injury, adult rats have decreased ability of continuous learning and memory and anxiety. This may be related to endoplasmic reticulum stress in hippocampus and cortex after spinal cord injury.
【作者单位】：重庆医科大学附属第二医院康复医学科;第三军医大学大坪医院野战外科研究所第三研究室创伤与烧伤国家重点实验室;
【基金】：国家自然科学基金面上项目(81272159) 重庆市教委科研项目(KJ1500237)~~
【分类号】：R651.2

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