纳米银涂层胆管支架与普通聚四氟乙烯胆管支架拮抗支架阻塞沉积物的对照实验研究

发布时间：2018-06-13 01:44

本文选题：纳米银粒子 + 胆管支架　；参考：《山东中医药大学》2015年硕士论文

【摘要】：目的:探讨化学氧化还原法制备纳米银粒子涂层胆管支架的可行性,观察纳米银粒子涂层形态学表征和化学成分稳定性。建立梗阻性黄疸比格犬动物实验模型,进行纳米银粒子涂层胆管支架与普通聚四氟乙烯胆管支架体内拮抗支架沉积物对照实验,为纳米银粒子涂层胆管支架临床应用提供动物实验依据。方法:实验共分两部分。第一部分采用化学氧化还原法制备纳米银涂层胆管支架,应用扫描电镜、能谱分析检测胆管支架纳米银粒子涂层的厚度及化学成分的稳定性。第二部分动物实验。采用12只实验用健康比格犬为研究对象,建立梗阻性黄疸动物模型后随机分为2组,分别行胆管支架置入术。纳米银涂层胆管支架组(n=6)、普通聚四氟乙烯胆管支架(n=6)。术后第7,21天分别行实验室血液白细胞、肝功检查,ELISA法检测TNF-α、IL-6变化。术后21天处死实验动物,取出胆管支架和胆管壁组织,分别行:胆管壁组织HE染色病理学检查,扫描电镜(SEM)观察胆管支架内沉积物附着程度;超声振荡清除胆管支架沉积物并比较沉积物重量;红外光谱分析胆管支架内沉积物成分;X线能谱仪分析(EDS)观察术前术后纳米银涂层稳定性。成果:1.化学氧化还原法制备纳米银粒子涂层胆管支架,SEM和EDXA观察纳米银粒子涂层厚度在1.5-6μm之间,银离子(Ag+)成分单一。2.建立梗阻性黄疸动物实验模型,建模前纳米银粒子涂层胆管支架组和Teflon胆管支架组比较,白细胞(WBC)、谷丙转氨酶(ALT)、谷草转氨酶(AST)、总胆红素(TBIL)、直接胆红素(DBIL)水平组间比较差异无统计学意义(P0.05);肿瘤坏死因子α(TNF-α)浓度和白细胞介素6(IL-6)浓度组间比较差异无统计学意义(P0.05),各项指标符合建模要求。建模后上述2组实验结果组间比较同建模前一致。3.动物实验表明,胆管支架置入后,犬梗阻性黄疸模型血液检查白细胞、肝功、IL-6、TNF-α较术均前有明显下降;纳米银涂层胆管支架组在胆管壁炎症反应程度、支架内沉积物厚度、重量方面测定均优于普通胆管支架组;支架内沉积物成分含有胆红素、胆固醇、胆汁酸、胆红素钙;EDS检查术前、术后纳米银涂层成分未见明显差异。结论:化学氧化还原法制备胆管支架理化性质稳定,能满足纳米银粒子涂层胆管支架动物实验的要求。结扎比格犬胆总管建立梗阻性黄疸动物实验模型,成功率高,能够满足纳米银粒子涂层胆管支架拮抗沉积物的实验要求。纳米银粒子体内实验中,纳米银粒子涂层胆管支架拮抗胆管支架沉积物的效果明显优于普通聚四氟乙烯胆管支架。本课题组将进一步进行实验研究,以期尽早将纳米银涂层胆管支架应用于临床。
[Abstract]:Aim: to investigate the feasibility of preparation of silver nanoparticles coated bile duct scaffolds by chemical redox method, and to observe the morphological characterization and chemical composition stability of silver nanoparticles coating. An experimental model of obstructive jaundice was established in Beagle dogs. A control study was conducted between silver nanoparticles coated bile duct scaffolds and common PTFE bile duct stents. To provide the animal experimental basis for the clinical application of silver nanoparticles coated biliary stent. Methods: the experiment was divided into two parts. In the first part, nano-silver coated bile duct scaffolds were prepared by chemical redox method. The thickness of silver nanoparticles coating and the stability of chemical composition were measured by scanning electron microscope and energy dispersive analysis. The second part is animal experiment. Twelve healthy Beagle dogs were used to establish obstructive jaundice model and were randomly divided into two groups. Nano-silver coated bile duct stent group (n = 6), ordinary PTFE bile duct stent (n ~ (6). The changes of TNF- 伪 and IL-6 were detected by Elisa on the 7th day after operation. The experimental animals were killed 21 days after operation. The bile duct stents and bile duct wall tissues were removed. The HE staining and SEM were used to observe the adhesion degree of the bile duct stents. Ultrasonic oscillation was used to remove the stents and the weight of the sediments was compared. The stability of nano-silver coatings before and after operation was observed by infrared spectroscopy (IR) and X-ray energy spectrometer (EDS). Result: 1. SEM and EDXA observation of Silver nanoparticles coated Biliary Stent by Chemical Redox method. The thickness of silver nanoparticles coating is between 1.5-6 渭 m. An animal model of obstructive jaundice was established and compared with Teflon bile duct stent group and nano-silver coated bile duct stent group before modeling. There was no significant difference in WBCU, alt, AST, TBILL, DBIL, TNF- 伪 and IL-6 between the WBC group and the control group (P > 0.05). There was no significant difference between the two groups in the levels of TBILA, TBILL and DBIL. There was no significant difference between the two groups in the concentration of tumor necrosis factor 伪 (TNF- 伪) and interleukin-6 (IL-6). The significance is P0.05, and the indexes meet the requirements of modeling. After modeling, the comparison between the above two groups of experimental results is consistent with that before modeling. 3. 3. Animal experiments showed that the leukocyte and IL-6TNF- 伪 levels in the blood of obstructive jaundice model were significantly decreased after biliary stent implantation, and the degree of inflammation in bile duct wall and the thickness of stents sediment in nano-silver coated bile duct stent group were significantly lower than those before operation. The content of bilirubin, cholesterol, bile acid, calcium bilirubin and calcium bilirubin in the sediments of the stents were better than those in the common bile duct stents before and after EDS examination, there was no significant difference in the composition of the nano-silver coatings after operation. Conclusion: the physical and chemical properties of bile duct scaffolds prepared by chemical redox method are stable and can meet the requirements of nano-silver particles coated bile duct stents. The animal model of obstructive jaundice was established by ligating the common bile duct of Beagle canine. In vivo experiments, silver nanoparticles coated bile duct scaffolds were more effective than conventional PTFE bile duct stents in antagonizing bile duct stents. Further experimental study will be carried out in order to apply nano-silver coated biliary stent to clinic as soon as possible.
【学位授予单位】：山东中医药大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R657.4

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