胶质细胞源性神经营养因子对股骨头缺血坏死的保护作用

发布时间：2018-06-15 13:37

本文选题：激素性股骨头坏死 + 胶质细胞源性神经营养因子　；参考：《中国骨质疏松杂志》2017年06期

【摘要】：目的探究胶质细胞源性神经营养因子(glial cell line-derived neurotrophic factor,GDNF)对早期激素性股骨头坏死(steroid-induced avascular necrosis of the femoral head,SANFH)的保护作用。方法采用改良技术建立早期激素性股骨头坏死兔模型,随机分成实验组(n=12)和对照组(n=12)。实验组给予髋周臀大肌注射GDNF(8.0μg/只),对照组相同部位注射等体积生理盐水,干预1周。分别于第5、10、15、20天进行股骨头多排螺旋CT、显微CT及苏木素-伊红(HE)染色检测。结果影像学观察示,GDNF注射后第15天开始,实验组股骨头骨小梁逐渐丰富、密集,间距变窄,骨皮质厚度及密度增加;对照组股骨头骨小梁分布稀疏,间距增宽,部分断裂,骨质密度减低。第15、20天,实验组与对照组股骨头骨矿物质密度、组织骨矿物质密度及骨体积分数,比较差异具有统计学意义(P0.05)。组织学观察示,实验组股骨头内骨小梁粗大,骨细胞分布广泛,细胞核着色深,脂肪细胞分布较少;对照组骨小梁分布稀疏,形态不完整,部分断裂,骨细胞空陷窝和脂肪细胞增多,骨细胞和造血细胞减少。第15、20天,实验组与对照组股骨头骨细胞空陷窝率分别为:13.3±3.2%、10.6±2.4%和71.0±7.5%、78.6±5.3%,两组比较,实验组骨细胞空陷窝率显著减低(P0.05)。结论 GDNF可通过抑制骨细胞坏死,保持骨小梁的完整性。
[Abstract]:Objective to investigate the protective effect of glial cell line-derived neurotrophic factor (GDF) on steroid-induced avascular necrosis of the femoral head (SANFH) in early steroid-induced femoral head necrosis. Methods the model of early steroid-induced femoral head necrosis in rabbits was established by modified technique and randomly divided into two groups: the experimental group and the control group. The experimental group was injected with GDNF 8.0 渭 g / rat, and the control group was injected with the same volume of normal saline for 1 week. Multislice spiral CT, microCT and hematoxylin-eosin HEH staining were performed on 15 ~ 20 days of 5 ~ (th) ~ 10 ~ (th) ~ (th) day of femoral head. Results from the 15th day after GDNF injection, the trabeculae of femoral head in the experimental group became richer, denser and narrower, the thickness and density of the bone cortex increased, and the trabeculae of the femoral head in the control group were distributed sparsely, the spacing was widened, and some fractures were observed. Bone density decreased. The bone mineral density, tissue bone mineral density and bone volume fraction of the femoral head in the experimental group and the control group were significantly higher than those in the control group (P 0.05). Histological observation showed that the trabeculae in the femoral head in the experimental group were thick, the bone cells were widely distributed, the nuclei were deeply stained, and the adipocytes were less, while the bone trabeculae in the control group were sparsely distributed, incomplete in shape and partially broken. The number of empty fossa and adipocytes increased, and the number of bone cells and hematopoietic cells decreased. On the 15th and 20th day, the empty recess rates of bone cells in the experimental group and the control group were respectively 10. 6 卤2. 4% and 71. 0 卤7. 5%, respectively. Compared with the control group, the rate of empty bone cells in the experimental group was significantly lower than that in the control group (10. 06 卤2. 4% and 71. 0 卤7. 5%). Conclusion GDNF can maintain the integrity of trabecular bone by inhibiting osteonecrosis.
【作者单位】：重庆医科大学附属第一医院放射科;成都医学院第一附属医院放射科;
【基金】：国家临床重点专科建设项目([2013]544) 四川省教育厅基金资助项目(15ZB0243)
【分类号】：R681.8

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