同种带瓣主动脉去内皮处理的基础研究

发布时间：2018-06-21 22:52

本文选题：主动脉瓣 + 同种移植　；参考：《青岛大学》2015年硕士论文

【摘要】：目的比较去内皮同种带瓣主动脉与传统去细胞同种瓣的生物学特性和免疫原性,探究性能更优越的同种瓣预处理方法。方法获取家兔带瓣主动脉根部组织,随机分为新鲜组(获取后无特殊处理,作为空白对照组)、去内皮细胞组(应用胶原酶处理法脱去内皮细胞)、去全部细胞组(应用传统方法脱去组织中全部的细胞成分)。应用HE、Masson、VG染色法及扫描电镜进行组织形态学观察,通过对比测定各组组织厚度、抗张强度、断裂拉伸率及热皱缩温度进行生物学特性评价,通过CD152免疫组织化学染色观察及原子火焰吸收法测定移植物钙含量变化进行免疫原性评价。结果组织形态学观察结果提示:去内皮和去细胞处理均达到了理想的脱细胞目的。三种组织的厚度、热皱缩温度无统计学差异(P0.05)。与新鲜组相比,去内皮细胞组抗张力性能变化不明显(P0.05),去全部细胞组抗张性能明显下降(P0.01)。新西兰兔皮下包埋实验后HE染色观察发现:新鲜组炎性反应最为剧烈,包埋后有大量炎细胞浸润;与新鲜组相比,去内皮细胞组包埋后炎性反应显著减弱,炎细胞数目明显减少,去全部细胞组炎性反应最弱,仅组织边缘有少量炎细胞浸润。免疫组化观察发现去内皮细胞组和去全部细胞组CD152表达水平明显高于对照组(P0.05),而去内皮细胞组与去全部细胞组之间未见显著差异(P0.05)。钙含量分析结果提示:去内皮细胞组和去全部细胞组包埋后钙盐增加量较新鲜组显著下降(P0.05),去内皮细胞组和去全部细胞组间未见统计学差异(P0.05)。结论同种带瓣主动脉经去内皮和传统去细胞处理后,免疫原性均显著下降,但去内皮处理较传统去细胞处理更好地保留了同种瓣的抗张性能。
[Abstract]:Objective to compare the biological characteristics and immunogenicity of endothelial homograft aortic homograft and traditional acellular aortic valve, and to explore the better preconditioning method of aortic homograft. Methods the rabbit aortic root tissue with valve was obtained and randomly divided into fresh group without special treatment. As a blank control group, the endothelial cells group (removal of endothelial cells by collagenase treatment) and the whole cell group (removal of all cell components in the tissue by traditional methods). The histomorphology of each group was observed by means of Masson's VG staining and scanning electron microscope. The biological characteristics of each group were evaluated by comparing the tissue thickness, tensile strength, tensile fracture rate and heat shrinkage temperature. The immunogenicity was evaluated by CD152 immunohistochemical staining and the determination of calcium content in grafts by atomic flame absorption spectrometry. Results the histomorphologic observation showed that both deendothelialization and acellular treatment had achieved the ideal acellular purpose. There was no significant difference in the thickness of the three tissues and the heat shrinkage temperature (P0.05). Compared with the fresh group, the tension resistance of the endothelial cells group did not change significantly (P0.05), the tensile performance of all the cells group decreased significantly (P0.01). The results of HE staining showed that the inflammatory reaction in the fresh group was the most intense, and a large number of inflammatory cells infiltrated after the embedding, and the inflammatory response in the endothelial cell removed group was significantly decreased compared with the fresh group. The number of inflammatory cells decreased significantly, and the inflammatory response was the weakest in all the cells group, with only a small number of inflammatory cells infiltrating at the edge of the tissue. Immunohistochemical observation showed that the expression of CD152 was significantly higher in the endothelium-free group and the total cell group than that in the control group (P0.05), but there was no significant difference between the endothelial cell removed group and the total cell removed group (P0.05). The results of calcium content analysis showed that the increase of calcium salt was significantly lower in the endothelium-free group and the total cell group than in the fresh group (P0.05), but there was no statistical difference between the endothelium-free group and the total cell group (P0.05). Conclusion the immunogenicity of aortic homograft was significantly decreased after both the removal of endothelium and the traditional decellularization of aorta, but the anti-tensile properties of the homograft were better preserved after the removal of endothelium than that of the conventional acellular treatment.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R654

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