珊瑚羟基磷灰石联合浓缩生长因子修复兔颅骨缺损

发布时间：2018-06-28 16:13

  本文选题：珊瑚羟基磷灰石 + 浓缩生长因子　； 参考：《医学研究生学报》2017年04期

【摘要】：目的骨缺损修复是临床一大难题,异质骨材料与生长因子复合以提高骨缺损修复能力近年来成为研究热点。文中旨在探讨异质骨材料中珊瑚羟基磷灰石(CHA)联合浓缩生长因子(CGF)修复骨缺损的效果。方法选24只新西兰兔,随机数字表法分为CHA/CGF+CHA组、CHA/CGF+自体骨组和CHA+自体骨组,每组8只,在每只兔的两侧颅骨上各制备1个直径10mm圆形缺损。术后6周和12周取材,通过Micro-CT及组织学观察评价骨修复效果。结果 Micro-CT结果显示,CHA/CGF+CHA组:术后6周骨缺损区CHA孔隙中有较多的新骨形成;术后12周CHA孔隙中有大量的新骨形成,骨密度明显增加,CHA部分降解吸收。CHA/CGF+自体骨组:术后6周邻近骨床的CHA孔隙内有少量新生骨长入;术后12周CHA孔隙内的新骨量明显增加。CHA+自体骨组:术后6周自体骨与骨床交界处有骨痂形成,边界模糊;术后12周自体骨与周围骨床完全融合,边界不清。CHA/CGF+CHA组术后6周和12周新骨量多于CHA+自体骨组[(39.00±7.61)mm3vs(32.12±6.55)mm3,(49.75±2.36)mm3vs(39.45±7.02)mm3,P0.05],骨密度多于CHA+自体骨组[(308.30±29.82)mg/m L vs(256.85±151.25)mg/m L,(389.00±31.87)mg/m L vs(302.53±127.05)mg/m L,P0.05],在术后12周,CHA/CGF+CHA组新骨量和骨密度与CHA+自体骨组差异无统计学意义(P0.05)。组织学观察结果:CHA+自体骨组术后6周植入的自体骨与骨床之间有新骨形成,并有少量结缔组织长入;术后12周,自体骨与周围骨床完全融合,边界不清。CHA/CGF+CHA组术后6周骨缺损区CHA孔隙内有散在的新骨形成;术后12周,CHA孔隙内有大量新骨形成并相互融合,部分CHA降解吸收。CHA/CGF+自体骨组术后6周近骨床的CHA孔隙中有少量编织骨长入;术后12周,新生骨质及类骨质面积增大,但缺损中央区域的CHA孔隙内仅有结缔组织充填,无新骨形成,CHA部分降解吸收。结论 CHA与CGF联合使用能有效地促进骨愈合,CHA/CGF复合人工骨是理想的骨移植替代材料。
[Abstract]:Objective Bone defect repair is a difficult problem in clinic. Heterogeneous bone materials combined with growth factor to improve the ability of bone defect repair has become a hot topic in recent years. The purpose of this paper is to investigate the effect of coralline hydroxyapatite (cha) combined with concentrated growth factor (CGF) in repairing bone defects in heterogeneous bone materials. Methods Twenty-four New Zealand rabbits were randomly divided into cha / CGF autogenous bone group and cha autogenous bone group with 8 rabbits in each group. A diameter 10mm circular defect was prepared on each side of the skull of each rabbit. The bone repair effect was evaluated by Micro-CT and histological observation at 6 and 12 weeks after operation. Results Micro-CT results showed that in CHA / CGF cha group, there were more new bone formation in cha pores in the defect area 6 weeks after operation, and a large number of new bone formation in cha pores at 12 weeks after operation. Bone mineral density increased significantly in cha partial degradation and absorption. Cha / CGF autogenous bone group: a small amount of new bone appeared in cha pore near the bone bed 6 weeks after operation. The volume of new bone in cha pore increased significantly at 12 weeks after operation. In cha autogenous bone group, callus formed at the junction of autogenous bone and bone bed 6 weeks after operation, and the boundary was blurred, 12 weeks after operation, autogenous bone was completely fused with surrounding bone bed. 杈圭晫涓嶆竻.CHA/CGF CHA缁勬湳鍚，

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