木犀草素抗骨关节炎的作用机制研究

发布时间：2018-06-29 20:33

本文选题：木犀草素 + 骨关节炎　；参考：《苏州大学》2015年博士论文

【摘要】：骨关节炎(osteoarthritis,OA),又称退行性骨关节病,是一种多发生于中老年人并以关节软骨的变性、破坏及骨质增生为特征的慢性关节病。木犀草素是一种天然黄酮类化合物,存在于多种蔬菜和药用植物中,其药理活性涉及抗氧化、抗炎、抗肿瘤、神经保护以及对血管的作用等方面。本文从整体动物和细胞水平层面上,探讨了木犀草素抗骨关节炎的作用机制,论文分为以下两个部分:第一部分木犀草素抗骨关节作用机制研究采用弗氏佐剂建立大鼠骨关节炎来评价木犀草素抗骨关节炎的作用,通过检测相关指标,包括脚肿胀体积、关节指数、血及滑膜液中白细胞及中性粒细胞数值、血清中炎症因子白介素-6(IL-6)、白介素-1β(IL-1β)、肿瘤坏死因子α(TNF-α)、Th1/Th2平衡、Th17/Treg平衡及P2X4信号通路并来评价木犀草素的抗骨关节炎作用,结果显示,木犀草素各剂量组均能显著减低骨关节炎大鼠脚肿胀体积,关节炎指数,血及滑膜液中白细胞及中性粒细胞数值,血清中炎症因子IL-6、IL-1β、TNF-α,调节Th1/Th2平衡、Th17/Treg平衡,抑制P2X4信号通路,结果显示木犀草素具有抗骨关节炎作用。第二部分木犀草素抗骨关节炎的细胞机制研究采用LPS-诱导巨噬细胞激活模型来评价木犀草素抗炎作用,通过检测相关指标,包括NO,IL-6,IL-1β,TNF-α,i NOS,MAPK/NF-кB信号通路P2X4信号通路,结果显示,木犀草素够抑制LPS-诱导巨噬细胞激活,说明木犀草素能对抗炎症的发生,从而抗骨关节炎。
[Abstract]:Osteoarthritis (OA), also known as degenerative osteoarthropathy, is a chronic arthropathy characterized by degeneration, destruction and hyperosteogenesis of articular cartilage. Luteolin is a kind of natural flavonoids found in many kinds of vegetables and medicinal plants. Its pharmacological activities are related to antioxidant, anti-inflammatory, anti-tumor, neuroprotective and vascular effects. In this paper, the mechanism of luteolin against osteoarthritis was studied on the level of whole animal and cell. The thesis is divided into two parts as follows: the first part is the mechanism of luteolin anti-osteoarthritis in rats. The effect of luteolin on osteoarthritis was evaluated by using Freund's adjuvant to evaluate the effect of luteolin on osteoarthritis. Joint index, leukocytes and neutrophils in blood and synovial fluid, serum inflammatory cytokines interleukin-6 (IL-6), interleukin-1 尾 (IL-1 尾), tumor necrosis factor- 伪 (TNF- 伪), Th1 / Th2 balance Th17 / Treg balance and P2X4 signaling pathway were used to evaluate the anti-osteoarthritis effect of luteolin. The results showed that luteolin could significantly reduce the swelling volume of foot, arthritis index, leukocyte and neutrophils in blood and synovial fluid, IL-6, IL-1 尾 and TNF- 伪, and regulate Th1 / Th2 balance and Th17 / Treg balance in rats with osteoarthritis. The inhibitory effect of luteolin on osteoarthritis was found by inhibiting P2X 4 signaling pathway. The second part of the study on the cellular mechanism of luteolin anti-osteoarthritis was to evaluate the anti-inflammatory effect of luteolin by using LPS-induced macrophage activation model. The results showed that luteolin could be used to evaluate luteolin's anti-inflammatory effect by detecting some related indexes, including the signal pathway P2X4 of NO-IL-6, IL-1 尾, TNF- 伪 and NOSN-MAPK / NF-B signaling pathway. Luteolin can inhibit LPS- induced macrophage activation, suggesting that luteolin can prevent inflammation and thus osteoarthritis.
【学位授予单位】：苏州大学
【学位级别】：博士
【学位授予年份】：2015
【分类号】：R684.3

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