成血管因子和溶骨因子在皂泡型和溶骨型骨巨细胞瘤中表达差异的分析

发布时间：2018-07-02 22:30

本文选题：骨巨细胞瘤 + 血管内皮生长因子　；参考：《中国矫形外科杂志》2017年07期

【摘要】：[目的]比较骨巨细胞瘤(giant cell tumor of bone,GCT)标本在各临床分级以及溶骨型和皂泡型中成血管和溶骨因子表达水平的差异,为GCT的临床治疗研究提供理论基础。[方法]选取2000年6月~2015年2月来自天津医院、济南军区总医院及河北省第三医院的GCT病例40例,其中典型皂泡征组20例,溶骨型组20例。利用免疫组化的方法检测所有标本VEGF(vascular endothelial growth factor)、CD34、PCNA(proliferating cell nuclear antigen)及RANKL(receptor activator of nuclear factor ligand)的表达,分别按照Campanacci和Ennecking临床分级以及皂泡型和溶骨型分组进行统计分析。[结果]VEGF和PCNA表达在Ennecking和Campanacci不同分级中差异无统计学意义(P0.05);CD34和RANKL在Campanacci不同分级中差异无统计学意义(P0.05),而在Ennecking分级中差异有统计学意义(χ~2=11.552,11.979;P=0.021,0.018);PCNA、RANKL及CD34表达在皂泡型组和溶骨型组之间差异无统计学意义(P0.05);VEGF在皂泡型组表达多于溶骨组,差异有统计学意义(χ~2=11.882;P=0.003)。[结论]VEGF、CD34、PCNA和RANKL在GCT的表达水平与其临床分级无完全平行的对应关系,皂泡型与溶骨型GCT在肿瘤细胞增殖和肿瘤对骨破坏情况上无明显区别,皂泡型与溶骨型GCT在肿瘤血管形成情况上有明显区别,皂泡征型GCT血管形成多于溶骨型GCT。
[Abstract]:[objective] to compare the expression levels of vascularization and osteolytic factors in (giant cell tumor of giant cell tumor (GCT) specimens in different clinical grades, and to provide a theoretical basis for the clinical treatment of GCT. [methods] from June 2000 to February 2015, 40 cases of GCT from Tianjin Hospital, General Hospital of Jinan military region and third Hospital of Hebei Province were selected, including 20 cases of typical soap-bubble syndrome group and 20 cases of osteolytic type group. The expression of PCNA (proliferating cell nuclear antigen) and RANKL (receptor activator of nuclear factor ligand) in all specimens were detected by immunohistochemical method. According to Campanacci and Ennecking clinical grade and soap-bubble type and osteolytic type, the expression of PCNA (proliferating cell nuclear antigen) and RANKL (receptor activator of nuclear factor ligand) were statistically analyzed. [results] the expression of VEGF and PCNA in different grades of Ennecking and Campanacci had no significant difference (P0.05). There was no significant difference between CD34 and RANKL in different grades of Campanacci (P0.05), but there was a significant difference in the expression of VEGF and PCNA in different grades of Campanacci (P < 0.05). The expression of VEGF in the soap-bubble group was higher than that in the osteolytic group (P0.05). The difference was statistically significant (蠂 2 + 11.882 P < 0.003). [conclusion] the expression levels of PCNA and RANKL in VEGF CD34 and RANKL in GCT have no completely parallel relationship with their clinical grade, but there is no significant difference between soap bubble type and osteolytic type in the proliferation of tumor cells and the destruction of bone by tumor. There was a significant difference between soap bubble type and osteolytic type GCT in tumor angiogenesis. Soap bubble type GCT angiogenesis was more than osteolytic type GCT.
【作者单位】：天津医科大学;天津市西青医院骨一科;天津市天津医院脊柱二科;天津市天津医院骨肿瘤科;中国骨巨细胞瘤协作组(GTOC);
【基金】：天津市卫生局课题(编号:15KG124) 天津市西青医院院级课题目(编号:xqyylx201606)
【分类号】：R738.1

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