鞣酸在骨关节炎中软骨保护作用的实验研究

发布时间：2018-07-05 14:17

本文选题：骨关节炎 + 软骨细胞　；参考：《中国矫形外科杂志》2017年11期

【摘要】：[目的]探究鞣酸(GA)在晚期糖基化终末产物(AGEs)诱导的骨关节炎中对软骨细胞的保护作用。[方法]首先通过应用晚期糖基化终末产物处理实验动物兔软骨细胞探索AGEs对于软骨细胞的负面作用;然后通过对离体软骨细胞代谢过程中活性氧、II型胶原蛋白、蛋白聚糖、一氧化氮合成酶和环氧化酶-2的测定,以及兔血清COMP和TNF-α的测定,观察GA在经AGEs处理的软骨细胞中的软骨保护作用。[结果]暴露于AGEs作用下的离体实验动物兔软骨细胞中,II型胶原蛋白和蛋白聚糖水平下降,而活性氧、一氧化氮合成酶、环氧化酶-2以及兔血清COMP和TNF-α水平升高,变化差异有统计学意义;相比之下经GA预处理的软骨细胞暴露于AGEs后,活性氧的释放仅有微弱的增加;AGEs介导的II型胶原和蛋白聚糖的降解可以被GA所阻断;由AGEs介导的i NOS和COX-2的表达受到抑制,兔血清COMP和TNF-α水平同时被GA所抑制。[结论]鞣酸可以在AGEs介导的骨关节炎中对动物软骨细胞起到保护作用,从而作为一种潜在的药物治疗方法延缓骨关节炎的进展。
[Abstract]:[objective] to investigate the protective effect of tannin (GA) on chondrocytes in advanced glycation end products (ages)-induced osteoarthritis. [methods] the negative effects of ages on chondrocytes were explored by treating rabbit chondrocytes with advanced glycation end products, and then reactive oxygen species type II collagen, proteoglycan, were used in the metabolism of chondrocytes in vitro. The protective effect of GA on chondrocytes treated with ages was observed by the determination of nitric oxide synthase (NOS) and cyclooxygenase-2 (COX-2), and the determination of comp and TNF- 伪 in rabbit serum. [results] the levels of type II collagen and proteoglycan in rabbit chondrocytes exposed to ages decreased, while reactive oxygen species (Ros), nitric oxide synthase (NOS), cyclooxygenase-2 (COX-2), and serum levels of comp and TNF- 伪 increased. In contrast, the release of reactive oxygen species only slightly increased the degradation of type II collagen and proteoglycan mediated by ages in the chondrocytes pretreated with GA. The expression of iNOS and COX-2 mediated by ages was inhibited, and the levels of comp and TNF- 伪 in rabbit serum were inhibited by GA. [conclusion] tannin can protect chondrocytes from osteoarthritis mediated by ages, which may be a potential drug therapy to delay the progress of osteoarthritis.
【作者单位】：北京德尔康尼骨科医院;首都医科大学附属北京朝阳医院;
【基金】：2015年度首都医科大学附属北京朝阳医院青年科研基金项目(编号:2015007)
【分类号】：R684.3

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