腺病毒介导TGFβ1基因转染半腱肌腱重建对兔ACL术后生物力学的影响

发布时间：2018-07-05 14:49

本文选题：前交叉韧带 + 腱骨愈合　；参考：《中国矫形外科杂志》2017年06期

【摘要】：[目的]探讨腺病毒介导TGFβ1基因转染半腱肌腱重建对兔ACL术后腱骨愈合的生物力学影响。[方法]新西兰兔48只,随机分为A、B、C三组,A组自体同侧转染Ad TGFβ1的半腱肌腱;B组自体同侧转染Ad GFP的半腱肌腱;C组自体同侧半腱肌腱DMEM处理,以兔双侧后肢单股半腱肌腱转染成功后重建ACL,术后第2周、4周、8周、12周处死取材,检测生物力学特性变化。[结果]A、B组半腱肌腱转染后,均可见绿色荧光表达;A组转染12 h时半腱肌腱组织中TGF-β1蛋白表达量为(221.0±12.2)ng/ml;每组两两比较,术后第2周,A、B、C三组移植物分别出现7例、6例、7例骨隧道中拔出,1例、2例、1例撕裂,差异无统计学意义(P0.05);术后第4周,A、B、C三组肌腱移植物分别出现6例、6例、7例骨隧道中拔出,2例、2例、1例撕裂,差异无统计学意义(P0.05);术后第8周,A、B、C三组移植物分别出现1例、6例、6例骨隧道中拔出,7例、2例、2例撕裂,差异有统计学意义(P0.05);术后第12周,A、B、C三组移植物分别出现1例、2例、2例骨隧道中拔出,7例、6例、6例撕裂,差异无统计学意义(P0.05)。术后第2周,每组最大拉伸载荷差异无统计学意义(P0.05);术后第4周、8周、12周A组的最大载荷较B、C组明显增高,差异有统计学意义(P0.05),对比之下,A组及B组差异无统计学意义(P0.05);随时间的推移,每组的刚度增加,但是组间差异均无统计学意义(P0.05)。术后第2周,每组最大拉伸载荷差异无统计学意义(P0.05);术后第4周、8周、12周A组的最大载荷较B、C组明显增高,差异有统计学意义(P0.05),对比之下,A组及B组差异无统计学意义(P0.05);随时间的推移,每组的刚度增加,但是组间差异均无统计学意义(P0.05)。[结论]腺病毒介导TGFβ1基因体外转染对自体半腱肌腱重建兔ACL术后早期可提供腱骨界面强大的抗拉强度,对膝关节早期康复有重要的意义。
[Abstract]:[objective] to investigate the biomechanical effects of adenovirus mediated TGF 尾 1 gene transfer on tendon healing after ACL in rabbits. [methods] Forty-eight New Zealand rabbits were randomly divided into three groups: group A: autogenous ipsilateral transfection of Ad TGF- 尾 1 semitendinosus tendon, group B: autogenous ipsilateral transfection of Ad GFP semitendinosus tendon, group C autogenous ipsilateral semitendinosus tendon DMEM treatment. ACLs were reconstructed with unilateral semitendinosus tendon of bilateral hind limbs after transfection. After 2 weeks, 4 weeks, 8 weeks and 12 weeks after operation, the specimens were sacrificed and the biomechanical characteristics were detected. [results] the expression of TGF- 尾 1 protein in semitendinosus tendon of group A was (221.0 卤12.2) ng / ml at 12 h after transfection of semitendinosus tendon. In the second week after operation, 7 cases of bone tunnel were found in 7 cases and 6 cases of bone tunnel were found in the three groups of bone grafts. One case was pulled out of the bone tunnel in 2 cases, 2 cases had tears, and 1 case had tears. There was no significant difference between the three groups (P0.05), 6 cases of tendon grafts appeared in 6 cases and 7 cases of bone tunnel were found in the 3 groups at the 4th week after operation, 2 cases were pulled out of bone tunnel, 2 cases had tears, 2 cases had tears. There was no significant difference between the three groups (P0.05), in the 8th week after operation, 1 case (6 cases) and 6 cases (6 cases) of bone tunnel were found in the three groups of bone grafts, 7 cases (7 cases) were pulled out of the bone tunnel, 2 cases (2 cases) had tears, and 2 cases (2 cases) had tears. The difference was statistically significant (P0.05), and there was no significant difference among the 3 groups (1 case, 2 cases, 2 cases, bone tunnel, 7 cases) and 6 cases (6 cases), the difference was not statistically significant (P0.05). At the second week after operation, there was no significant difference in the maximum tensile load in each group (P0.05), but the maximum load in group A was significantly higher than that in group B C at the 4th week and 12th week after operation. The difference was statistically significant (P0.05). There was no significant difference between group A and group B (P0.05). With the passage of time, the stiffness of each group increased, but there was no significant difference between groups (P0.05). At the second week after operation, there was no significant difference in the maximum tensile load in each group (P0.05), but the maximum load in group A was significantly higher than that in group B C at the 4th week and 12th week after operation. The difference was statistically significant (P0.05). There was no significant difference between group A and group B (P0.05). With the passage of time, the stiffness of each group increased, but there was no significant difference between groups (P0.05). [conclusion] Adenovirus-mediated TGF 尾 1 gene transfection in vitro can provide a strong tensile strength at the interface of tendon and bone in the early stage of reconstruction of rabbit ACL with autologous semitendinosus tendon, which is of great significance for early rehabilitation of knee joint.
【作者单位】：南华大学附属第二医院关节外科;
【基金】：湖南省教育厅资助项目(编号:13c836)
【分类号】：R687.4

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