S-烯丙基巯基半胱氨酸对乳腺癌细胞裸鼠移植瘤的抑瘤作用及其机制

发布时间：2018-07-08 13:37

本文选题：S-烯丙基巯基半胱氨酸 + 乳腺癌　；参考：《山东医药》2017年39期

【摘要】：目的观察S-烯丙基巯基半胱氨酸(SAMC)对乳腺癌细胞裸鼠移植瘤的抑瘤作用,并探讨其作用机制。方法将MCF-7细胞悬液接种于BALB/C雌性裸鼠右侧腋下近上肢处成瘤,制作乳腺癌MCF-7细胞裸鼠皮下移植瘤模型。将造模成功的裸鼠随机分为对照组、低剂量SAMC组、高剂量SAMC组、紫杉醇组。对照组给予Nacl注射液,低剂量SAMC组给予150 mg/kg的SAMC,高剂量SAMC组给予300 mg/kg的SAMC,紫杉醇组给予10 mg/kg的紫杉醇。每周腹腔注射给药3次,共给药3周。末次给药后第2天处死裸鼠,取移植瘤组织称重,计算各组肿瘤抑瘤率;HE染色镜下观察肿瘤组织病理变化;采用免疫组化法检测各组肿瘤组织中的VEGF、Caspase-3蛋白。结果低剂量SAMC组、高剂量SAMC组、紫杉醇组瘤重低于对照组(P均0.05)。高剂量SAMC组肿瘤抑制率高于低剂量SAMC组(P0.05),但与紫杉醇组相比差异无统计学意义。肿瘤组织HE染色见低剂量SAMC组、高剂量SAMC组、紫杉醇组出现不同程度的细胞坏死,三组坏死面积和程度依次增大。对照组、低剂量SAMC组、高剂量SAMC组、紫杉醇组肿瘤组织中Caspase-3蛋白相对表达量依次升高,VEGF相对表达量依次降低(P均0.05)。结论 SAMC可抑制乳腺癌细胞裸鼠移植瘤的生长,机制可能与Caspase-3蛋白表达上调、VEGF蛋白表达下调有关。
[Abstract]:Objective to investigate the inhibitory effect of Sallyl mercapto cysteine (SAMC) on breast cancer xenografts in nude mice and its mechanism. Methods MCF-7 cell suspension was inoculated into the right subaxillary proximal upper limb of BALB / C female nude mice to establish tumor model of breast cancer in nude mice. The successful nude mice were randomly divided into control group, low dose SAMC group, high dose SAMC group and paclitaxel group. The control group was given Nacl injection, the low dose SAMC group was given 150 mg/kg SAMC, the high dose SAMC group was given 300 mg/kg SAMC, and the paclitaxel group was given 10 mg/kg paclitaxel. Three times a week for 3 weeks. The nude mice were killed on the second day after the last administration, the tumor tissue was weighed, the tumor inhibition rate was calculated and the pathological changes of tumor tissue were observed by HE staining, and the VEGF- Caspase-3 protein in tumor tissue was detected by immunohistochemical method. Results the tumor weight of low dose SAMC group, high dose SAMC group and paclitaxel group was lower than that of control group (P 0.05). The tumor inhibition rate of high dose SAMC group was higher than that of low dose SAMC group (P0.05), but there was no significant difference compared with paclitaxel group. He staining showed that there were different degrees of cell necrosis in low dose SAMC group, high dose SAMC group and paclitaxel group, and the necrotic area and degree of the three groups increased in turn. The relative expression of Caspase-3 protein in tumor tissues of control group, low dose SAMC group, high dose SAMC group and paclitaxel group decreased in turn (P 0.05). Conclusion SAMC can inhibit the growth of breast cancer xenografts in nude mice. The mechanism may be related to the up-regulation of Caspase-3 protein expression and down-regulation of VEGF protein expression.
【作者单位】：华北理工大学附属唐山市人民医院;
【分类号】：R737.9

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