硫酸软骨素调配海藻酸钠复合胶对兔骨折愈合的影响
发布时间：2018-07-24 16:05
【摘要】：目的探讨海藻酸钠复合胶中加入硫酸软骨素对兔骨折愈合的影响。方法新西兰大白兔72只,适应性喂养1 w,随机分为胶1组、胶2组、丝线组和模型组,每组18只。无菌手术条件下在桡骨上造成一大小为0.8 cm×0.4 cm蝶形骨块,其中胶1组用A胶(海藻酸钠∶羧甲基纤维素钠:硫酸软骨素=6.0∶2.5∶0.75)黏接固定,胶2组用B胶(海藻酸钠∶羧甲基纤维素钠=6.0∶2.5)黏接固定,并在黏接处滴饱和氯化钙溶液5滴,使胶表面形成一层海藻酸钙膜;丝线组以4号丝线将骨块捆扎固定;模型组将骨块放至原位置,不做任何固定;逐层缝合切口,用无菌敷料包扎。术后2 w、4 w和6 w分别对胶1组、胶2组、丝线组、模型组拍摄X线片,观察骨折愈合情况,然后各组于各时间段处死6只取出骨折端进行骨痂组织切片HE染色,镜下观察不同时间段各组骨痂生长情况;采集骨折局部皮下组织,酶联免疫吸附试验(ELISA)检测不同时间段各组骨折局部皮下组织中骨形态发生蛋白(BMP)、白细胞介素(IL)-6含量。结果术后X线片:胶1组、胶2组术后6 w骨折骨性愈合,位线良好;丝线组骨折线基本消失,骨干外形成陈旧性骨折影;模型组骨折畸形愈合,可见明显骨缺损。HE染色:胶1组、胶2组术后骨软细胞成骨活跃,炎症反较轻,丝线组和模型组肉芽组织增生明显,炎症反应较重。术后2、4、6 w时骨折局部皮下组织中IL-6、BMP的ELISA检测:骨折局部皮下组织中BMP的含量均为胶1组胶2组丝线组模型组(P0.05);IL-6的水平以丝线组最高(P0.05),胶1组、胶2组和模型组之间无统计学差异(P0.05)。结论海藻酸钠和羧甲基纤维素钠的复合胶能有效黏合小骨折块,炎症反应小;硫酸软骨素能促进骨折愈合过程中BMP的表达,在海藻酸钠和羧甲基纤维素钠的复合胶中加入一定比例的硫酸软骨素制成的新型骨黏合剂更有利于骨折的愈合。
[Abstract]:Objective to investigate the effect of adding chondroitin sulfate into sodium alginate compound glue on fracture healing in rabbits. Methods 72 New Zealand white rabbits were fed adaptively for 1 week. They were randomly divided into three groups: glue group (n = 18), silk thread group (n = 18) and model group (n = 18). A butterfly bone mass of 0.8 cm 脳 0.4 cm in size was created on the radius under aseptic operation condition, in which group 1 was glued and fixed with A glue (sodium alginate: sodium carboxymethyl cellulose: chondroitin sulfate 6.0: 2.5: 0.75). Group 2 was fixed with B glue (sodium alginate: sodium carboxymethyl cellulose 6.0: 2.5) and saturated calcium chloride solution (5 drops) was dripped on the adhesive to form a layer of calcium alginate film on the surface of glue. In the model group, the bone was placed in its original position without any fixation. The incision was sutured layer by layer and wrapped with aseptic dressing. At 4 and 6 weeks after operation, X-ray films were taken from glue 1 group, glue 2 group, silk thread group and model group, respectively. The healing of fracture was observed, and then 6 rats were killed at each time point to be stained with HE in callus tissue sections. Bone morphogenetic protein (BMP),) interleukin (IL) -6 (IL) -6) in local subcutaneous tissue of fracture was detected by (ELISA). Results X-ray films: the fracture bone union was good in group 1 and group 2 at 6 weeks after operation, the fracture line of silk thread group disappeared basically, the old fracture shadow formed outside the backbone, the fracture malunion of model group was observed. Obvious bone defect. HE staining: bone soft cell osteogenesis was active and inflammation was lighter in group 1 and group 2, granulation tissue proliferation and inflammatory reaction were more serious in silk thread group and model group. ELISA detection of IL-6 BMP in local subcutaneous tissue of fracture at 2 weeks and 4 weeks postoperatively: the content of BMP in local subcutaneous tissue of fracture was the highest (P0.05) in the silk thread group and the highest in the glue 1 group (P0.05), and the level of IL-6 was the highest in the glue group (P0.05), and the level of IL-6 in the glue group was the highest (P0.05), the level of IL-6 in the glue group was the highest (P0.05). There was no statistical difference between glue 2 group and model group (P0.05). Conclusion the composite adhesive of sodium alginate and sodium carboxymethyl cellulose can effectively bind to the small fracture block and the inflammatory reaction is small, and chondroitin sulfate can promote the expression of BMP during fracture healing. Adding a certain proportion of chondroitin sulfate to the composite adhesive of sodium alginate and sodium carboxymethyl cellulose is more beneficial to fracture healing.
【作者单位】： 厦门市第二医院;福建中医药大学;厦门市中医院;
【基金】：福建省卫生教育联合攻关项目(WKJ2008-2-38)
【分类号】：R683
，
本文编号：2141901
[Abstract]:Objective to investigate the effect of adding chondroitin sulfate into sodium alginate compound glue on fracture healing in rabbits. Methods 72 New Zealand white rabbits were fed adaptively for 1 week. They were randomly divided into three groups: glue group (n = 18), silk thread group (n = 18) and model group (n = 18). A butterfly bone mass of 0.8 cm 脳 0.4 cm in size was created on the radius under aseptic operation condition, in which group 1 was glued and fixed with A glue (sodium alginate: sodium carboxymethyl cellulose: chondroitin sulfate 6.0: 2.5: 0.75). Group 2 was fixed with B glue (sodium alginate: sodium carboxymethyl cellulose 6.0: 2.5) and saturated calcium chloride solution (5 drops) was dripped on the adhesive to form a layer of calcium alginate film on the surface of glue. In the model group, the bone was placed in its original position without any fixation. The incision was sutured layer by layer and wrapped with aseptic dressing. At 4 and 6 weeks after operation, X-ray films were taken from glue 1 group, glue 2 group, silk thread group and model group, respectively. The healing of fracture was observed, and then 6 rats were killed at each time point to be stained with HE in callus tissue sections. Bone morphogenetic protein (BMP),) interleukin (IL) -6 (IL) -6) in local subcutaneous tissue of fracture was detected by (ELISA). Results X-ray films: the fracture bone union was good in group 1 and group 2 at 6 weeks after operation, the fracture line of silk thread group disappeared basically, the old fracture shadow formed outside the backbone, the fracture malunion of model group was observed. Obvious bone defect. HE staining: bone soft cell osteogenesis was active and inflammation was lighter in group 1 and group 2, granulation tissue proliferation and inflammatory reaction were more serious in silk thread group and model group. ELISA detection of IL-6 BMP in local subcutaneous tissue of fracture at 2 weeks and 4 weeks postoperatively: the content of BMP in local subcutaneous tissue of fracture was the highest (P0.05) in the silk thread group and the highest in the glue 1 group (P0.05), and the level of IL-6 was the highest in the glue group (P0.05), and the level of IL-6 in the glue group was the highest (P0.05), the level of IL-6 in the glue group was the highest (P0.05). There was no statistical difference between glue 2 group and model group (P0.05). Conclusion the composite adhesive of sodium alginate and sodium carboxymethyl cellulose can effectively bind to the small fracture block and the inflammatory reaction is small, and chondroitin sulfate can promote the expression of BMP during fracture healing. Adding a certain proportion of chondroitin sulfate to the composite adhesive of sodium alginate and sodium carboxymethyl cellulose is more beneficial to fracture healing.
【作者单位】： 厦门市第二医院;福建中医药大学;厦门市中医院;
【基金】：福建省卫生教育联合攻关项目(WKJ2008-2-38)
【分类号】：R683
，
本文编号：2141901
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