多种脊髓损伤致继发性肠道功能障碍的模型比较

发布时间：2018-07-28 13:00

【摘要】：目的通过3种不同方法制备大鼠脊髓损伤致继发性肠道功能障碍的模型,探究各组脊髓损伤致继发性肠道功能障碍模型的表现差别,为脊髓损伤继发肠道功能障碍的相关研究提供稳定的实验动物模型复制方法和实验基础。方法将48只SD大鼠随机分为打击组(A组,n=12)、全横断组(B组,n=12)、钳夹组(C组,n=12)、假手术组(D组,n=12)。于术后1 d、3d、7 d分别对各组进行BBB功能评分;肠肌电慢波测定;血清D-乳酸含量测定;HE染色观察肠组织形态。结果术后1 d、3d、7 d,A、B、C组BBB评分、肌电慢波振幅均低于D组(P0.05);Chiu肠黏膜评分、血清D-乳酸浓度均显著高于D组(P0.05)。术后第7天,A组血清D-乳酸浓度和Chiu肠黏膜评分与B、C两组间差异存在显著性(P0.05)。结论不同方法制备脊髓损伤后,各组均继发肠道功能障碍,其中A组模型复制较为稳定,且死亡率较低,更适用于周期较长的实验研究。
[Abstract]:Objective to establish the model of secondary intestinal dysfunction induced by spinal cord injury in rats by three different methods, and to explore the difference of secondary intestinal dysfunction model caused by spinal cord injury in each group. To provide a stable experimental animal model replication method and experimental basis for the related research of secondary intestinal dysfunction after spinal cord injury. Methods Forty-eight Sprague-Dawley rats were randomly divided into three groups: group A (n = 12), group B (n = 12), group C (n = 12) and group D (n = 12). BBB score, slow wave measurement of intestinal electromyography, and serum D- lactic acid content were measured on the 7th day after operation, and the intestinal morphology was observed by HE staining. Results the BBB score and the amplitude of EMG slow wave in group C were lower than those in group D (P0.05), and the serum levels of D-lactic acid were significantly higher than those in group D (P0.05). On the 7th day after operation, there were significant differences in serum D- lactic acid concentration and Chiu intestinal mucosal score between group A and group C (P0.05). Conclusion after the spinal cord injury was prepared by different methods, all the groups had secondary intestinal dysfunction. The model of group A was more stable, and the mortality was lower, which was more suitable for the experimental study with a longer period.
【作者单位】：成都医学院基础医学实验教学中心;
【基金】：四川省教育厅2016年度科研计划重点项目(16ZA0296) 成都医学院实验室开放项目(kf2016003)
【分类号】：R-332;R651.2

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