罗哌卡因对皮肤损伤愈合及GM-CSF表达的影响

发布时间：2018-08-02 16:48

【摘要】：目的观察罗哌卡因对皮肤损伤小鼠皮肤愈合及粒细胞-巨噬细胞集落刺激因子(GM-CSF)表达的影响。方法将小鼠随机分为实验组(罗哌卡因)和对照组(生理盐水),两组小鼠创面进行Masson染色,测定GM-CSF浓度和羟脯氨酸水平,记录创面的愈合率和愈合时间。结果第5天,实验组绿染胶原纤维沉积量较对照组多;第10天,实验组较对照组创缘上皮增生明显。实验组小鼠第5天GM-CSF含量高于对照组(P0.05)。实验组小鼠创面愈合时间短于对照组(P0.05)。实验组大鼠第5天和第10天创面愈合比例高于对照组(P0.05)。实验组大鼠第5天和第10天创面羟脯氨酸水平高于对照组(P0.05)。结论罗哌卡因局部浸润可以促进小鼠全层皮肤损伤的愈合,其机制可能与局部浸润罗哌卡因能够促进GM-CSF的表达有关。
[Abstract]:Objective to observe the effects of ropivacaine on skin healing and granulocyte-macrophage colony stimulating factor (GM-CSF) expression in mice with skin injury. Methods mice were randomly divided into two groups: the experimental group (ropivacaine) and the control group (normal saline). Masson staining was performed on the wound surface of the two groups. The concentration of GM-CSF and hydroxyproline were measured, and the healing rate and healing time of the wound were recorded. Results on the 5th day, the deposition of green stained collagen fibers in the experimental group was more than that in the control group, and on the 10th day, the hyperplasia of the wound edge epithelium was more obvious in the experimental group than in the control group. The content of GM-CSF in the experimental group was higher than that in the control group on the 5th day (P0.05). The wound healing time in the experimental group was shorter than that in the control group (P0.05). The rate of wound healing on the 5th and 10th day in the experimental group was higher than that in the control group (P0.05). The level of hydroxyproline in the experimental group was higher than that in the control group on the 5th and 10th day (P0.05). Conclusion Local infiltration of ropivacaine can promote the healing of skin injury in mice, and the mechanism may be related to the role of ropivacaine in promoting the expression of GM-CSF.
【作者单位】：浙江省人民医院;
【基金】：浙江省自然科学基金(No:LY15H090014)
【分类号】：R614

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