右美托咪定对谷氨酸所致PC12细胞损伤的保护作用及其机制

发布时间：2018-08-09 15:52

【摘要】：目的研究右美托咪定(Dex)对谷氨酸所致PC12细胞损伤的保护作用及机制。方法应用高浓度谷氨酸处理PC12细胞以建立细胞缺氧损伤模型。应用MTT法测定细胞存活率,采用试剂盒分别测定细胞培养液中乳酸脱氢酶释放量,细胞丙二醛含量和超氧化物歧化酶活力,DCFH-DA染色流式细胞仪检测细胞内活性氧水平,Fluo-8染色流式细胞仪检测细胞内钙离子含量,JC-1染色流式细胞仪检测细胞线粒体膜电位。结果在0.01~100μmol/L浓度范围内,Dex浓度依赖性地拮抗谷氨酸所致PC12细胞损伤,至100μmol/L时,细胞存活率达到正常组的(86.6±2.2)%,显著高于模型组(P0.01),乳酸脱氢酶释放量为正常组的1.4±0.1倍,显著低于模型组(P0.01)。1μmol/L Dex预处理谷氨酸损伤的PC12细胞,与模型组相比,能够显著降低丙二醛含量(P0.01),提高超氧化物歧化酶活力(P0.01),抑制胞内活性氧过度生成(P0.01),降低细胞内Ca2+浓度(P0.01),稳定细胞线粒体膜电位(P0.01)。结论 Dex对谷氨酸所致PC12细胞损伤具有保护作用,其机制可能与Dex抗氧化和抑制细胞内钙超载,保护线粒体功能相关。
[Abstract]:Objective to study the protective effect and mechanism of dexmetomidine (Dex) on glutamate induced PC12 cells. Methods PC12 cells were treated with high concentration of glutamate to establish anoxic injury model. The cell survival rate was measured by MTT assay, and the lactate dehydrogenase release from cell culture medium was measured by kit. The content of malondialdehyde (MDA) and the activity of superoxide dismutase (SOD) were detected by DCFH-DA staining flow cytometry. The intracellular calcium content was detected by Fluo-8 staining flow cytometry and the mitochondrial membrane potential was detected by JC-1 staining flow cytometry. Results in the concentration range of 0.01 渭 mol/L, Dex antagonized the damage of PC12 cells induced by glutamate in a concentration-dependent manner. At 100 渭 mol/L, the cell survival rate was (86.6 卤2.2), significantly higher than that in the model group (P0.01), and the release of lactate dehydrogenase was 1.4 卤0.1 times of that in the normal group. It was significantly lower than that in model group (P0.01). 1 渭 mol/L Dex pretreated PC12 cells damaged by glutamate, compared with model group. It could significantly decrease the content of malondialdehyde (P0.01), increase the activity of superoxide dismutase (P0.01), inhibit the excessive production of intracellular reactive oxygen species (P0.01), decrease the concentration of intracellular Ca2 (P0.01), and stabilize the mitochondrial membrane potential (P0.01). Conclusion Dex has protective effect on PC12 cells induced by glutamate, and its mechanism may be related to the antioxidation of Dex, inhibition of intracellular calcium overload and protection of mitochondrial function.
【作者单位】：解放军总医院麻醉手术中心;军事医学科学院卫生与环境研究所;
【基金】：国家973项目(2012CB518200) 军队“十二五”重大专项(AWS11J003)~~
【分类号】：R614

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