葛根素治疗急性脊髓缺血再灌注损伤大鼠的神经保护机制

发布时间：2018-08-17 17:34

【摘要】：目的探索葛根素在治疗急性脊髓缺血再灌注损伤(ASII)大鼠中的作用机制。方法 28只雄性大鼠阻断主动脉来实施ASII。实验动物随机分为4组:阴性对照(NC)组(假手术处理)、阳性对照(IR+S)组(脊髓缺血再灌注损伤+盐水处理)、IR+P组(脊髓缺血再灌注损伤+葛根素处理)、IR+R组(脊髓缺血再灌注损伤+细胞周期蛋白B激酶抑制剂处理)。检测各组大鼠运动机能、脊髓梗死体积、细胞凋亡指数及细胞周期素依赖性激酶5(CDK5)和P25的活性。结果 IR+S组与NC组比较,P35和P25蛋白表达差异有统计学意义(P0.01);IR+P组、IR+R组与IR+S组比较差异亦有统计学意义(P0.05)。提示葛根素对ASII神经保护可能通过类似抑制剂的作用,并且与P35和P25的表达相关。结论脊髓损伤减小与CDK5及P25活性受抑制相关;在ASII大鼠治疗过程中,抑制CDK5及P25活性是葛根素的神经保护机制之一。
[Abstract]:Objective to explore the mechanism of puerarin in the treatment of acute spinal cord ischemia reperfusion injury in (ASII) rats. Methods the aorta of 28 male rats was blocked to perform ASII. The experimental animals were randomly divided into four groups: negative control (NC) group (sham-operation treatment), positive control (IR S) group (spinal cord ischemia-reperfusion injury saline treatment) and IR P group (puerarin treatment) and IR R group (spinal cord). Treatment with cyclin B kinase inhibitor in medullary ischemia-reperfusion injury). Motor function, infarct volume, apoptosis index, cyclin dependent kinase 5 (CDK5) and P25 activity were measured. Results the expression of P35 and P25 protein in IR S group was significantly different from that in NC group (P0.01). There was also significant difference between IR P group and IR S group (P0.05). These results suggest that puerarin may play a role in the neuroprotection of ASII through similar inhibitors, and may be related to the expression of P35 and P25. Conclusion the decrease of spinal cord injury is related to the inhibition of CDK5 and P25 activities, and the inhibition of CDK5 and P25 activities is one of the neuroprotective mechanisms of puerarin during the treatment of ASII rats.
【作者单位】：中国医科大学附属盛京医院骨外科;
【基金】：辽宁省教育厅科学技术研究项目(l2015589)
【分类号】：R651.2

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