胞质型磷脂酶A2与核因子κB在单肺通气致兔肺损伤中的相互作用

发布时间：2018-09-03 07:04

【摘要】：目的探讨胞质型磷脂酶A2(C-PLA2)与核因子κB(NF-κB)间相互作用关系,阐明单肺通气上调C-PLA2表达的作用机制。方法 48只健康日本大耳白兔随机均分为8组:对照组;溶剂预处理组;NF-κB抑制剂(PDTC)/溶剂预处理组;C-PLA2抑制剂(AACOCF3)/溶剂预处理组;单肺通气组;溶剂预处理+单肺通气组;NF-κB抑制剂(PDTC)/溶剂预处理+单肺通气组和CPLA2抑制剂(AACOCF3)/溶剂预处理+单肺通气组。Western-blot和定量PCR分别用于检测肺组织C-PLA2和NF-κB蛋白及m RNA表达水平;ELISA检测肺组织花生四烯酸含量;支气管-肺泡灌洗液嗜中性粒细胞计数、肺组织髓过氧化物酶活性、肺干/湿重比值和肺组织学评分用于反映肺损伤的严重程度。结果单纯给予溶剂未对实验动物造成明显副作用。给予AACOCF3或PDTC,但未行单肺通气的实验动物除C-PLA2或NF-κB明显下调外(P0.05),其余指标无显著变化。单肺通气实验动物肺组织C-PLA2和NF-κB表达水平均显著上调(P0.05),出现明显的肺损伤(P0.05);AACOCF3或PDTC预处理均可显著降低单肺通气实验动物肺组织C-PLA2和NF-κB表达水平(P0.05),同时肺损伤程度明显减轻(P0.05)。结论 C-PLA2和NF-κB在单肺通气致肺损伤中均发挥重要作用,二者相互调控,相互影响。
[Abstract]:Objective to investigate the interaction between cytoplasmic phospholipase A 2 (C-PLA2) and nuclear factor- 魏 B (NF- 魏 B), and to elucidate the mechanism of up-regulation of C-PLA2 expression by single lung ventilation. Methods Forty-eight healthy Japanese white rabbits were randomly divided into 8 groups: control group, (PDTC) group with NF- 魏 B inhibitor / solvent pretreatment group, C-PLA2 inhibitor (AACOCF3) / solvent pretreatment group, single lung ventilation group; The expression of C-PLA2 and NF- 魏 B protein and m RNA in lung tissue were detected by (PDTC) / AACOCF3 / AACOCF3 / PCR, respectively. The content of arachidonic acid in lung tissue was detected by Elisa. The number of neutrophils in bronchoalveolar lavage fluid (BALF), myeloperoxidase activity in lung tissue, lung dry / wet weight ratio and lung histological score were used to reflect the severity of lung injury. Results only given solvent did not cause obvious side effects on experimental animals. The experimental animals which were given AACOCF3 or PDTC, but no single lung ventilation had no significant change except C-PLA2 or NF- 魏 B (P0.05). The expression of C-PLA2 and NF- 魏 B were significantly up-regulated (P0.05), and the expression of C-PLA2 and NF- 魏 B were significantly decreased (P0.05), and the degree of lung injury was significantly alleviated (P0.05). Conclusion both C-PLA2 and NF- 魏 B play an important role in lung injury induced by single lung ventilation.
【作者单位】：昆明医科大学医学机能实验中心;云南省第一人民医院麻醉科;
【基金】：云南省教育厅一般项目(2014C027Y) 云南省科技厅-昆明医科大学联合专项(2014FB-098) 云南省卫生科技计划项目(2014NS244)
【分类号】：R61

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