七氟醚预处理在肾缺血再灌注中的保护作用

发布时间：2018-10-16 10:49

【摘要】：目的:探讨七氟醚(Sev)预处理减轻肾缺血再灌注损伤的作用与HIF-2α表达的关系。方法:将野生型(WT)小鼠和HIF-2α基因敲除(HIF-2α-/-)小鼠随机分为假手术组(Control组)、肾缺血再灌注组(I/R组)和Sev预处理+肾缺血再灌注组(I/R+Sev组)。建立肾缺血再灌注模型后检测肾血流量、血尿素氮(BUN)和肌酐(Cr)的水平,TUNEL法检测肾脏细胞凋亡,Western blotting检测肾组织HIF-2α水平。结果:WT小鼠中I/R+Sev组的血BUN和Cr水平显著低于I/R组(P0.001)、肾相对血流量显著高于I/R组(P0.05)、细胞凋亡数明显少于I/R组(P0.05),而HIF-2α-/-小鼠中I/R组和I/R+Sev组的这些监测指标都没有统计学差异。WT小鼠中I/R+Sev组的HIF-2α蛋白水平显著高于I/R组(P0.05)。结论 :Sev预处理可能通过增加HIF-2α表达的稳定性,从而抑制细胞凋亡、改善肾功能,达到肾保护作用。
[Abstract]:Aim: to investigate the effect of sevoflurane (Sev) preconditioning on renal ischemia reperfusion injury and the expression of HIF-2 伪. Methods: wild type (WT) mice and HIF-2 伪 knockout (HIF-2 伪-/ -) mice were randomly divided into three groups: sham operation group (Control group), renal ischemia reperfusion group (I / R group) and Sev preconditioning renal ischemia reperfusion group (I / R Sev group). Renal blood flow, blood urea nitrogen (BUN) and creatinine (Cr) levels were measured after renal ischemia-reperfusion model, and renal apoptosis was detected by TUNEL, Western blotting. Results: the serum BUN and Cr levels in the I / R Sev group were significantly lower than those in the I / R group (P0.001), and the renal relative blood flow was significantly higher in the WT mice than in the I / R group (P0.05), and the number of apoptosis was significantly lower than that in the I / R group (P0.05). However, there was no uniform monitoring index in the HIF-2 伪-/-group I / R and I / R Sev group. The level of HIF-2 伪 protein in I / R Sev group was significantly higher than that in I / R group (P0.05). Conclusion: Sev pretreatment may inhibit apoptosis, improve renal function and achieve renal protection by increasing the stability of HIF-2 伪 expression.
【作者单位】：上海交通大学医学院附属仁济医院南院麻醉科;上海交通大学附属仁济医院东院麻醉科;
【基金】：上海市卫生局科研课题基金资助项目(编号:20124059)
【分类号】：R614

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