急性创伤应激大鼠QT间期、Cx43变化及其与室性心律失常的关系

发布时间：2018-10-23 17:02

【摘要】：目的:通过研究急性创伤应激(acute traumatic stress,ATS)大鼠QT间期、心脏缝隙连接蛋白43(Connexion 43,Cx43)的变化规律及其与室性心律失常(ventricular arrhythmia,VA)的关系,为ATS后VA的防治提供实验依据。方法:(1)64只SD大鼠,采用左后肢截肢方式建立ATS模型,分别于术后不同时间点(0min、3min、10min、30min、45min、60min、90min),各处死8只大鼠,即实验分为术时组、术后0~3min组、术后3~10min组、术后10~30min组、术后30~45min组、术后45~60min组、术后60~90min组及术前对照组共8组。(2)ATS模型全程II导联心电图监测,观察心率、PR间期、QT间期,心率校正QT间期(QTc)的变化规律及VA发生情况,同时对各时段行VA评分。(3)HE染色观察对比各组大鼠心肌细胞形态结构变化情况。(4)免疫组织化学染色观察心肌细胞Cx43蛋白分布及水平变化情况。结果:(1)各组心率存在显著差异。与术前对照组相比,大鼠截肢后心率普遍增快,术后0~3min心率增快最明显,为390.39±37.19次/min(p0.05),此后逐渐下降;(2)各组QT间期、QTc间期存在显著差异。与对照组相比,截肢后早期QT及QTc间期缩短,最短均发生于术后0~3min,分别为0.41±0.07s及0.147±0.022s(p0.05),随后逐渐恢复;(3)截肢后50%(28/56)的大鼠出现VA,大鼠各时段VA的发生率、VA评分存在统计学差异(p0.05),截肢后0~3min,VA的发生率及VA的评分最高,分别为35.4%,0.44±0.741。(4)HE染色结果显示,与术前对照组相比,截肢后各组大鼠心肌结构无明显变化。(5)免疫组织化学结果显示,截肢术后0min、3min、10min、30min、45min心肌细胞Cx43分布紊乱,以截肢后3min时改变最为明显,同时Cx43蛋白水平在3min时呈下降趋势,而在60min后趋于正常。结论:VA主要发生在ATS早期且严重程度最高,ATS早期VA发生的同时QT间期、QTc间期缩短,Cx43分布紊乱、蛋白水平呈下降趋势。
[Abstract]:Objective: to study the relationship between QT interval, gap junction protein 43 (Connexion 43) and ventricular arrhythmia (ventricular arrhythmia,VA) in acute traumatic stress (acute traumatic stress,ATS) rats, and to provide experimental evidence for the prevention and treatment of VA after ATS. Methods: (1) ATS models were established in 64 SD rats by left hind limb amputation. Eight rats were killed at different time points (0 min, 3 min, 10 min, 30 min, 45 min, 60 min, 90 min). The rats were divided into three groups: operation time group, postoperative 0~3min group, postoperative 3~10min group, postoperative 10~30min group, postoperative 30~45min group and postoperative 45~60min group. There were 8 groups in 60~90min group and control group before operation. (2) ECG monitoring of II lead in the whole course of ATS model. The changes of heart rate, PR interval, QT interval, (QTc) of heart rate correction QT interval and the occurrence of VA were observed. At the same time, VA score was used in each period. (3) HE staining was used to observe and compare the morphological and structural changes of cardiac myocytes in each group. (4) immunohistochemical staining was used to observe the distribution and level of Cx43 protein in cardiac myocytes. Results: (1) there were significant differences in heart rate among groups. Compared with the control group, after amputation, the heart rate of rats increased generally, and the heart rate of 0~3min increased most obviously after amputation, which was 390.39 卤37.19 times / min (p0.05), and then decreased gradually. (2) there were significant differences in QT interval and QTc interval in each group. Compared with the control group, the QT and QTc intervals in the early stage of amputation were shorter than those in the control group. The shortest occurred at 0 ~ 3 min after amputation, 0.41 卤0.07 s and 0.147 卤0.022 s (p0.05) respectively, and then recovered gradually. (3) 50% (28 / 56) of the rats after amputation had the incidence of VA in each stage, the VA score was statistically different (p0.05), the incidence of VA and VA score were the highest at 0.3 min after amputation. (4) the results of HE staining showed that there was no significant change in myocardial structure after amputation compared with the control group. (5) the immunohistochemical results showed that the distribution of Cx43 was disordered at 0 min, 3 min, 10 min, 30 min and 45 min after amputation, especially at 3min after amputation. At the same time, the level of Cx43 protein decreased in 3min, but tended to normal after 60min. Conclusion: VA mainly occurs at the early stage of ATS, and the severity is the highest. At the same time, the QT interval, QTc interval is shortened, Cx43 distribution is disordered, and the protein level is decreasing while VA occurs in the early stage of ATS.
【学位授予单位】：桂林医学院
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R541.7;R641

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