小口径人工血管置换手术的兔实验模型构建
发布时间：2018-10-24 21:08
【摘要】：目的构建出能够更好的模拟冠心病搭桥术后桥血管血流动力学改变的动物实验模型。方法 16只新西兰大白兔随机分为两组。利用4 mm内径,长度为3 cm的膨体聚四氟乙烯血管置换兔的腹主动脉,切口位置分别选择在腹主中下1/3切口和下腹部切口,对比两组动物的基本情况、手术的时间、需要结扎的侧支血管数量、术中大出血的几率、术后血管吻合后的波动情况及血管通畅几率,并完善手术的注意事项和术后的动物处理方法等。结果两组动物在体质量,腹主动脉直径无明显差异(P0.05)。在手术时间方面,选择切口在中下1/3组有一增长的趋势,但无统计学差异(P0.05)。切口选择在腹部中下1/3组在需要结扎的分支血管数量少于下腹部切口组,术后血管两端吻合口波动良好的比率大于下腹部切口组。两组在出血的几率,术后7 d的栓塞几率方面无明显差异(P0.05)。结论利用兔制备小口径血管的置换模型可行,可根据动物表现和彩超检查及时发现小口径血管通畅与否。
[Abstract]:Objective to establish an animal model of hemodynamic changes after coronary artery bypass grafting. Methods Sixteen New Zealand white rabbits were randomly divided into two groups. The abdominal aorta of rabbits was replaced with expanded PTFE vessels with 4 mm inner diameter and 3 cm in length. The incision position was selected in the middle and lower third incision and the lower abdominal incision, respectively. The basic condition and operation time of the two groups were compared. The number of collateral vessels that need to be ligated, the chance of massive bleeding during operation, the fluctuation of blood vessel anastomosis after operation and the probability of vessel patency, as well as the matters needing attention in operation and the treatment methods of animals after operation were improved. Results there was no significant difference in the body mass and the diameter of abdominal aorta between the two groups (P 0.05). In terms of operative time, there was an increasing trend of incision selection in the middle and lower 1 / 3 groups, but there was no statistical difference (P0.05). The number of branches needed to be ligated was less in the incision group than in the lower abdominal incision group, and the ratio of good fluctuation of anastomotic stoma between the two ends was higher than that in the lower abdominal incision group. There was no significant difference in the probability of bleeding and embolization 7 days after operation between the two groups (P0.05). Conclusion it is feasible to establish a small diameter vascular replacement model in rabbits, which can be found in time according to animal manifestation and color Doppler examination.
【作者单位】： 南方医科大学珠江医院胸心外科;南方医科大学南方医院胸心外科;
【基金】：广东省产学研重大专项引导项目(2012B091100159) 广东省科技计划项目(2013B021800156)
【分类号】：R-332;R654.2
，
本文编号：2292560
[Abstract]:Objective to establish an animal model of hemodynamic changes after coronary artery bypass grafting. Methods Sixteen New Zealand white rabbits were randomly divided into two groups. The abdominal aorta of rabbits was replaced with expanded PTFE vessels with 4 mm inner diameter and 3 cm in length. The incision position was selected in the middle and lower third incision and the lower abdominal incision, respectively. The basic condition and operation time of the two groups were compared. The number of collateral vessels that need to be ligated, the chance of massive bleeding during operation, the fluctuation of blood vessel anastomosis after operation and the probability of vessel patency, as well as the matters needing attention in operation and the treatment methods of animals after operation were improved. Results there was no significant difference in the body mass and the diameter of abdominal aorta between the two groups (P 0.05). In terms of operative time, there was an increasing trend of incision selection in the middle and lower 1 / 3 groups, but there was no statistical difference (P0.05). The number of branches needed to be ligated was less in the incision group than in the lower abdominal incision group, and the ratio of good fluctuation of anastomotic stoma between the two ends was higher than that in the lower abdominal incision group. There was no significant difference in the probability of bleeding and embolization 7 days after operation between the two groups (P0.05). Conclusion it is feasible to establish a small diameter vascular replacement model in rabbits, which can be found in time according to animal manifestation and color Doppler examination.
【作者单位】： 南方医科大学珠江医院胸心外科;南方医科大学南方医院胸心外科;
【基金】：广东省产学研重大专项引导项目(2012B091100159) 广东省科技计划项目(2013B021800156)
【分类号】：R-332;R654.2
，
本文编号：2292560
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