七氟醚后处理对1型糖尿病大鼠局灶性脑缺血再灌注后STAT3信号通路的影响

发布时间：2018-11-10 17:48

【摘要】：目的:通过复制1型糖尿病大鼠的脑缺血再灌动物模型,观察七氟醚后处理对糖尿病大鼠局灶性脑缺血再灌注损伤后STAT3信号通路的影响,并探讨其作用机制。方法:成年SD雄性大鼠适应性饲养及空腹12h后,腹腔注射链脲佐菌素(STZ)60mg/kg制备T1DM模型。按随机分组原则分为4组:假手术(Sham)组、缺血再灌注(IR)组、七氟醚后处理(Sevo)组、阻断剂(B)组,每组各10只。参照Zea Longa线栓法制备大鼠局灶性脑缺血再灌注模型,采用Longa评分法进行神经行为学评分,HE染色观察组织结构的病理变化,ELISA检测血清s-100β蛋白含量,Western blot检测stat3、p-stat3的表达情况,以及对脑梗面积进行测量。结果:与Sham组相比,IR组的神经行为学评分、梗死面积及血清s-100β蛋白含量明显升高(P0.01);与IR组相比,Sevo组及B组的神经行为学评分、梗死面积及血清s-100β蛋白含量明显降低(P0.05);Sevo组与B组比较各指标均无明显差异。在Stat3总蛋白表达量上,各组间差异无统计学意义(P0.05);而在磷酸化Stat3的表达方面与Stat3总蛋白类似,Sevo组与IR组的磷酸化Stat3表达量差异无统计学意义(P0.05)。与其余各组相比,B组磷酸化Stat3表达量明显下降,(P0.05)。结论:在1型糖尿病模型大鼠中,七氟醚后处理对缺血再灌注脑组织是有保护作用的,但这短期(24h)保护作用并非通过Stat3信号传导通路活化来完成的。
[Abstract]:Aim: to investigate the effect of sevoflurane post-treatment on STAT3 signal pathway after focal cerebral ischemia-reperfusion injury in type 1 diabetic rats. Methods: adult SD male rats were fed with streptozotocin (STZ) 60mg/kg) for 12 hours on an empty stomach. T1DM model was established by intraperitoneal injection of streptozotocin (STZ) 60mg/kg). According to the principle of random grouping, they were divided into 4 groups: sham operation (Sham) group, ischemia reperfusion (IR) group, sevoflurane post-treated (Sevo) group, and blocker (B) group, 10 rats in each group. The rat model of focal cerebral ischemia-reperfusion was established by Zea Longa thread embolization method. The neurobehavioral score was evaluated by Longa scoring method, the pathological changes of tissue structure were observed by HE staining, and the serum s-100 尾 protein content by ELISA was detected by, Western blot to detect stat3,. The expression of p-stat3 and the area of cerebral infarction were measured. Results: compared with Sham group, the neurobehavioral score, infarct size and serum s-100 尾 protein content in IR group were significantly increased (P0.01). Compared with IR group, the neurobehavioral score, infarct size and serum s-100 尾 protein content in Sevo and B groups were significantly lower than those in IR group (P0.05). There was no significant difference in each index between); Sevo group and B group. In the total protein expression of Stat3, there was no significant difference among the groups (P0.05), but the expression of phosphorylated Stat3 was similar to the total protein of Stat3. There was no significant difference in the expression of phosphorylated Stat3 between Sevo group and IR group (P0.05). Compared with other groups, the expression of phosphorylated Stat3 in group B was significantly decreased (P0.05). Conclusion: sevoflurane post-treatment has protective effect on cerebral ischemia-reperfusion in type 1 diabetic rats, but the short-term (24h) protective effect is not achieved by activation of Stat3 signal transduction pathway.
【学位授予单位】：暨南大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R614

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