组织工程复合支架聚乳酸-羟基乙酸共聚物和羟基磷灰石纳米粒子接种自体骨髓间充质干细胞应用于骨再生（英文）

发布时间：2018-11-13 07:54

【摘要】：目的:对应用接枝的羟基磷灰石(g-HA)/聚乳酸-羟基乙酸共聚物(PLGA)纳米复合支架接种自体骨髓间充质干细胞(MSCs)和骨形态发生蛋白2(BMP-2)治疗重症骨缺损的新的治疗策略进行评估,并通过肌肉内移植研究人工骨的组织相容性和移植物在体内的矿化和缺损骨的愈合。创新点:改性的PLGA接种自体MSCs的组织工程骨加速了骨缺损的愈合,使临床重症骨缺损的治疗有了新的手段。方法:应用溶剂浇铸和粒子沥滤方法将PLGA和g-HA制备成复合支架g-HA/PLGA。在g-HA/PLGA支架上接种兔自体MSCs制成组织工程移植物。取宽0.3 cm长2.0 cm的上述移植物埋入兔背部肌肉内,8周后取出移植物,使用扫描式电子显微镜(SEM)检测人工骨的组织相容性(图3a),X射线能量色散谱(EDX)分析钙浓度。然后,用锯锯掉兔前肢桡骨骨干2.0 cm,取同样长度的上述移植物放置于骨缺损处(图4)。术后2、4、8周应用计算机X线摄影(CR)检测骨缺损愈合情况(图5),组织学分析愈合组织结构(图6),SEM检测人工骨与周围组织的相容性(图7),反转录聚合酶链式反应(RT-PCR)检测愈合组织Collagen I、Collagen II和Bmp-2基因的表达。结论:PLGA掺入g-HA主要改善了矿化作用,有益于骨相关基因的表达和骨形成。自体MSCs的应用增强了骨形成和PLGA支架的矿化作用,并加速了支架的吸收。
[Abstract]:Objective: to treat autogenous bone marrow mesenchymal stem cells (MSCs) and bone morphogenetic protein 2 (BMP-2) by grafting hydroxyapatite (g-HA) / poly (lactic acid-glycolic acid) (PLGA) nanocomposite scaffolds. New strategies for the treatment of severe bone defects are evaluated, The histocompatibility of artificial bone, the mineralization of grafts in vivo and the healing of defect bone were studied by intramuscular transplantation. Innovation: tissue engineered bone with modified PLGA inoculated with autologous MSCs accelerates the healing of bone defect and provides a new method for the treatment of severe bone defect. Methods: PLGA and g-HA were prepared by solvent casting and particle leaching. Tissue engineering grafts were made by inoculating autologous MSCs onto g-HA/PLGA scaffold. The graft, which was cm in width, was implanted into the rabbit back muscle. The graft was removed after 8 weeks. The histocompatibility of the artificial bone was detected by scanning electron microscopy (SEM) (fig. 3a). The concentration of calcium was analyzed by X-ray energy dispersive spectroscopy (EDX). The grafts of the same length were then sawed off the radial diaphysis of the forelimb of the rabbit at 2. 0 cm, and placed at the bone defect (Fig. 4). Bone defect healing was detected by computerized radiography (CR) 8 weeks after operation (Fig. 5), and tissue structure was analyzed by histology (Fig. 6), SEM for the compatibility of artificial bone with surrounding tissues (Fig. 7). Reverse transcriptase polymerase chain reaction (RT-PCR) was used to detect the expression of II and Bmp-2 genes in the healing tissues. Conclusion: PLGA incorporation into g-HA can improve mineralization, and is beneficial to the expression of bone related genes and bone formation. The application of autologous MSCs enhanced bone formation and mineralization of PLGA scaffolds, and accelerated the absorption of the scaffolds.
【作者单位】： Department
【基金】：Project supported by the National Natural Science Foundation of China(Nos.51473164 and 51273195) the Joint Research Project of Chinese Academy of Sciences and Japan Society for the Promotion of Science(CAS-JSPS;No.GJHZ1519) the International Science and Technology Cooperation Program of China(No.2014DFG52510)
【分类号】：R318.08;R68

【相似文献】