不同时期局部移植人脐带间充质干细胞修复脊髓损伤的组织学观察

发布时间：2018-11-13 09:00

【摘要】：背景:脊髓损伤能造成各种运动、感觉功能障碍,肌张力异常及病理反射等,临床上已通过人脐带间充质干细胞移植对脊髓损伤进行治疗。目的:探讨大鼠脊髓损伤后不同时期局部移植人脐带间充质干细胞修复脊髓损伤的效果。方法:选取SPF级雄性成年SD大鼠100只,随机分为5组:空白对照组、单纯脊髓损伤组及3,7,21 d后移植组,各20只。后4组利用Allen’s打击器制作大鼠T10脊髓损伤模型;3,7,21 d后移植组大鼠分别于脊髓损伤后3,7和21 d在损伤部位周围移植人脐带间充质干细胞。结果与结论:与单纯脊髓损伤组相比,3,7,21 d后移植组大鼠造模后49 d运动功能评分增加,血清白细胞介素2水平减少,血清白细胞介素10水平增加,脊髓病理损伤明显改善,且7 d后移植组效果最为明显。说明人脐带间充质干细胞移植修复大鼠脊髓损伤,改善大鼠运动功能,且在大鼠脊髓损伤7 d后移植效果最佳。
[Abstract]:Background: spinal cord injury (sci) can cause various motility, sensory dysfunction, muscular dystonia and pathological reflex, and has been treated clinically by transplantation of human umbilical cord mesenchymal stem cells. Aim: to investigate the effect of local transplantation of human umbilical cord mesenchymal stem cells (HMSCs) on spinal cord injury (sci) after spinal cord injury (sci) in rats. Methods: 100 adult SPF male SD rats were randomly divided into 5 groups: blank control group, simple spinal cord injury group and transplantation group (n = 20). The model of T10 spinal cord injury was made by Allen's in the last 4 groups, and the mesenchymal stem cells of human umbilical cord were transplanted in the transplantation group on the 7th and 21st days after spinal cord injury. Results and conclusion: compared with the simple spinal cord injury group, the motor function score increased, the serum interleukin 2 level decreased and the serum interleukin 10 level increased in the transplantation group 21 days after transplantation. The pathological injury of spinal cord was improved obviously, and the effect of transplantation group was the most obvious 7 days later. The results showed that transplantation of human umbilical cord mesenchymal stem cells to repair spinal cord injury and improve motor function of rats was the best after 7 days of spinal cord injury in rats.
【作者单位】：南京医科大学附属江苏盛泽医院骨科;南京医科大学第一附属医院骨科;
【分类号】：R651.2

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