盐酸戊乙奎醚后处理对缺血再灌注大鼠心肌炎症反应的影响

发布时间：2018-11-17 07:56

【摘要】：目的探讨盐酸戊乙奎醚(PHC)后处理对缺血再灌注大鼠心肌炎症反应的影响,以及PHC后处理抗炎症反应的调控通路。方法 24只实验Wistar大鼠,按随机数字表法分为3组:假手术组(Sham组),大鼠冠状动脉左前降支穿线不结扎;缺血再灌注(I/R组)组,大鼠冠状动脉左前降支结扎30 min,再灌注3 h;PHC后处理组(Post组),再灌注即刻大鼠尾静脉注射PHC(1 mg/kg)。各组在3个不同的时间点抽血,采用ELISA法检测血清炎症因子[肿瘤坏死因子-α(TNF-α)和白细胞介素-8(IL-8)]含量。实验结束,采用Western blot的方法,检测炎症调节蛋白[核因子κB抑制蛋白α(IκB-α)和核因子-κB(NF-κB)]的表达。结果与Sham组比较,I/R组血清TNF-α和IL-8含量明显增加,心肌组织IκB-α表达减少,NF-κB表达增加(P0.05)。与I/R组比较,Post组血清TNF-α和IL-8含量明显减少,心肌组织IκB-α表达增加,NF-κB表达减少(P0.05)。结论 PHC后处理能够抑制心肌I/R导致的炎症反应,可能的机制是通过调控经典的NF-κB信号通路。
[Abstract]:Objective to investigate the effects of (PHC) treatment with pentanosine hydrochloride on myocardial inflammatory response in rats with ischemia reperfusion and the regulation pathway of anti-inflammatory response after PHC treatment. Methods Twenty-four experimental Wistar rats were randomly divided into three groups: sham operation group (Sham group) without ligation of left anterior descending branch of coronary artery. In the ischemia reperfusion group (I / R group), the left anterior descending coronary artery was ligated for 30 min, and reperfusion for 3 h (Post group). Immediately after reperfusion, PHC (1 mg/kg) was injected into the tail vein of the rats. The serum levels of inflammatory factor (TNF- 伪) and interleukin-8 (IL-8) were measured by ELISA method at three different time points in each group. At the end of the experiment, Western blot was used to detect the expression of inflammatory regulatory protein (I 魏 B inhibitor 伪 and nuclear factor- 魏 B (NF- 魏 B). Results compared with Sham group, serum TNF- 伪 and IL-8 levels in I / R group were significantly increased, I 魏 B- 伪 expression was decreased and NF- 魏 B expression was increased in myocardial tissue (P0.05). Compared with I / R group, serum TNF- 伪 and IL-8 levels in Post group were significantly decreased, I 魏 B- 伪 expression was increased and NF- 魏 B expression was decreased in myocardial tissue (P0.05). Conclusion PHC postprocessing can inhibit myocardial I / R induced inflammation by regulating the classical NF- 魏 B signaling pathway.
【作者单位】：长沙市第四医院麻醉科;首都医科大学;首都医科大学附属北京安贞医院麻醉中心;
【基金】：国家自然科学基金资助项目(编号:81471902)
【分类号】：R614

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