损伤软骨的细胞提
发布时间：2018-11-18 08:56
【摘要】：目的对从膝关节软骨损伤区分离出的细胞进行鉴定,并对其生长活性进行研究,以解决自体软骨细胞移植中软骨细胞来源不足的问题。方法通过膝关节镜,在软骨损伤区取少量损伤软骨组织,不含正常软骨及软骨下骨为实验组;取正常软骨组织为对照组。在胰蛋白酶和Ⅱ型胶原酶作用下从实验组中提取细胞,对照组中提取正常软骨细胞。实验组细胞培养贴壁后在倒置相差显微镜下观察细胞的形态是否与对照组软骨细胞一致。实验组细胞行甲苯胺蓝染色鉴定,与对照组对比;噻唑蓝(MTT)法比较两组细胞生长活性;以具有专利知识产权的Ⅰ/Ⅱ型复合胶原膜为生长载体,培养实验组和对照组第3代软骨细胞,在电镜下观察两组生长活性。结果从少量损伤的软骨组织中提取的细胞为活性正常的软骨细胞,其特征及生长活性与对照组比较,差异无统计学意义(P0.05)。结论软骨损伤过程中软骨细胞未发生变性,少量损伤的软骨组织仍可获得适量软骨细胞,MTT法和电镜扫描表明,其生长活性符合自体软骨细胞移植的要求,无需取正常软骨组织作为细胞来源。
[Abstract]:Objective to identify the cells isolated from cartilage injury area of knee joint and to study their growth activity in order to solve the shortage of chondrocytes in autologous chondrocyte transplantation. Methods through knee arthroscopy, a small amount of injured cartilage tissue was taken from the injured area of cartilage, without normal cartilage and subchondral bone as experimental group, and normal cartilage tissue was taken as control group. The normal chondrocytes were extracted from the experimental group and the control group under the action of trypsin and collagenase. The morphology of the cells in the experimental group was observed under inverted phase contrast microscope after adherent to the wall and was consistent with the chondrocytes of the control group. The cells of the experimental group were identified by toluidine blue staining and compared with the control group, and the growth activity of the two groups was compared by (MTT) assay. The third passage chondrocytes of the experimental group and the control group were cultured with the type 鈪，
本文编号：2339547
[Abstract]:Objective to identify the cells isolated from cartilage injury area of knee joint and to study their growth activity in order to solve the shortage of chondrocytes in autologous chondrocyte transplantation. Methods through knee arthroscopy, a small amount of injured cartilage tissue was taken from the injured area of cartilage, without normal cartilage and subchondral bone as experimental group, and normal cartilage tissue was taken as control group. The normal chondrocytes were extracted from the experimental group and the control group under the action of trypsin and collagenase. The morphology of the cells in the experimental group was observed under inverted phase contrast microscope after adherent to the wall and was consistent with the chondrocytes of the control group. The cells of the experimental group were identified by toluidine blue staining and compared with the control group, and the growth activity of the two groups was compared by (MTT) assay. The third passage chondrocytes of the experimental group and the control group were cultured with the type 鈪，
本文编号：2339547
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