Micro-CT观察重组人甲状旁腺素在骨折愈合过程中的作用

发布时间：2018-11-21 11:11

【摘要】：目的使用Micro-CT,探讨重组人甲状旁腺激素(rh PTH)在2月龄雄性大鼠骨折愈合过程中的作用。方法应用2月龄雄性SD大鼠建立单侧闭合性股骨骨折内固定模型,随机分为甲状旁腺素治疗组和对照组,术后分别皮下注射重组人甲状旁腺素(1-34)10μg/(kg.d)或等剂量生理盐水安慰剂(对照组),分别于术后第2、7、14、21和42天取双侧股骨标本,分别行X线检查和Micro-CT观察,并进行骨结构的定量分析。结果术后第21天,甲状旁腺素组骨折断端两侧的骨痂之间连续性良好,骨折线已模糊不清。甲状旁腺组较对照组,在术后第42天骨体积(bone Volume,BV)、骨体积分数(BV/TV)、骨密度(bone mineral density,BMD)和骨小梁模式因子(trabecular bone pattern factor,Tb.Pf)明显高于对照组;而骨小梁分离度(trabecular separation,Tb.Sp)和各向异性的程度(degree of anisotropy,DA)低于对照组。结论低剂量重组人甲状旁腺激素可提高大鼠骨折BV、BV/TV、Tb.Pf和BMD而降低Tb.Sp和DA,从而促进骨折的愈合。
[Abstract]:Objective to investigate the role of recombinant human parathyroid hormone (rh PTH) in fracture healing of 2 month old male rats by Micro-CT,. Methods two month old male SD rats were used to establish a unilateral closed femoral fracture model and were randomly divided into two groups: parathyroid hormone treatment group and control group. After operation, 10 渭 g / (kg.d) of recombinant human parathyroid hormone (1-34) or placebo (control group) were injected subcutaneously respectively. X-ray examination and Micro-CT observation were performed, and quantitative analysis of bone structure was carried out. Results on the 21st day after operation, the continuity of callus between the two sides of the fracture in parathyroid hormone group was good, and the fracture line was blurred. The bone volume (bone Volume,BV), bone volume fraction (BV/TV), bone mineral density (bone mineral density,BMD) and trabecular model factor (trabecular bone pattern factor,Tb.Pf) in parathyroid gland group were significantly higher than those in control group. The bone trabecular separation (trabecular separation,Tb.Sp) and anisotropic degree (degree of anisotropy,DA) were lower than those in the control group. Conclusion low dose recombinant human parathyroid hormone can increase BV,BV/TV,Tb.Pf and BMD, decrease Tb.Sp and DA, and promote fracture healing in rats.
【作者单位】：北京市创伤骨科研究所;北京积水潭医院创伤骨科;北京积水潭医院矫形骨科;
【基金】：国家自然科学基金(编号:81071499、81330043)
【分类号】：R683

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