鸸鹋股骨头骨坏死标本微观结构改变特征及机制研究
发布时间：2018-11-22 13:01
【摘要】：目的:建立一种能够模拟人类股骨头骨坏死早期表现的鸸鹋股骨头坏死模型,研究鸸鹋股骨头坏死标本中不同区域内的骨微观结构的特征,探讨股骨头骨坏死的原因,以寻求安全有效的临床保头治疗的方案。方法:以成年鸸鹋为研究对象,用局部注射无水乙醇的方法来建立股骨头骨坏死的动物模型,对股骨头标本进行Micro-CT扫描及病理学检测;根据骨质密度的不同,将每个标本分为骨质破坏区、骨质增生区、正常区,对不同区域的骨微观结构通过组织学检查、Micro-CT检查来观察比较;用实时荧光定量PCR对不同区域内成、破骨细胞活性进行观察分析。结果:1.通过局部注射无水乙醇建立鸸鹋骨股头骨坏死模型:术后实验动物无明显感染表现,未出现异常死亡病例。无股骨头塌陷,股骨头内出现不同程度的骨坏死表现。骨计量学结果表明,造模侧比对照侧骨小梁厚度(Tb.th)上升了87%,骨小梁间距(Tb.sp)降低了43%,骨矿化密度(BMD)和骨小梁数目(Tb.N)没有明显变化。2.Micro-CT检测表明,股骨头坏死标本中出现明显的区域化差异。骨质破坏区域的骨小梁出现骨折、连续性破坏;骨质增生区域骨小梁间隙变窄、数目增多;正常区的骨小梁厚度分布均匀,结构完整。骨质破坏区骨小梁的BMD、Tb.Th、Tb.N与正常区相比有不同程度的降低,Tb.Sp与正常区相比升高,差异有统计学意义(P0.05)。骨质增生区骨小梁的BMD、Tb.Th、Tb.N与正常区相比有不同程度的升高,Tb.Sp与正常区相比降低,差异有统计学意义(P0.05)。3.病理学结果表明,鸸鹋股骨头坏死早期标本各区域骨小梁微观构造存在明显不同之处。骨质破坏区骨小梁连续性破坏,骨质增生区骨小梁密度变大,排列致密。4.RT-PCR的结果表明,骨吸收相关基因RANKL和RANK在骨质破坏区表达高于骨质增生区及正常区;骨形成相关基因BMP2和OPG在骨质增生区及正常区表达高于骨质破坏区。结论:1.通过注射无水乙醇方法可以建立的鸸鹋股骨头坏死模型。2.鸸鹋股骨头坏死标本呈现出明显的分区化差异,骨质破坏区骨小梁密度变小,骨质增生区骨小梁密度变大。3.骨质增生区成骨细胞活性增强;骨质破坏区破骨细胞活性增强。
[Abstract]:Objective: to establish an emu model of femoral head necrosis which can mimic the early manifestation of human femoral head necrosis, to study the characteristics of bone microstructure in different areas of femoral head necrosis in emus, and to explore the causes of femoral head necrosis. In order to seek a safe and effective clinical head-keeping treatment. Methods: adult emus were used to establish the animal model of femoral head osteonecrosis by local injection of anhydrous alcohol. The femoral head specimens were examined by Micro-CT and pathology. According to the difference of bone density, each specimen was divided into bone destruction area, bone hyperplasia area and normal area. The microstructure of bone in different areas was observed and compared by histological examination and Micro-CT examination. The activity of osteoclasts in different regions was observed and analyzed by real-time fluorescence quantitative PCR. Results: 1. The osteonecrosis model of femoral head of emus was established by local injection of anhydrous ethanol. There was no collapse of the femoral head and various degrees of osteonecrosis appeared in the femoral head. The bone metrology results showed that the trabecular thickness (Tb.th) of the model side increased by 87% and the trabecular spacing (Tb.sp) decreased by 43% compared with the control side. There was no significant change in bone mineralization density (BMD) and bone trabecular number (Tb.N). 2.Micro-CT analysis showed that there were significant regional differences in femoral head necrosis specimens. The bone trabeculae in the bone destruction area appeared fracture, the continuity destruction; the bone trabecular space became narrow, the number increased in the hyperosteogenic area; the normal area bone trabecular thickness distribution is uniform, the structure is complete. The BMD,Tb.Th,Tb.N of trabecular bone in bone destruction area was lower than that in normal area, and Tb.Sp was higher than that in normal area (P0.05). The BMD,Tb.Th,Tb.N of bone trabeculae in hypertrophic area was higher than that in normal area, and Tb.Sp was lower than that in normal area (P0.05). The pathological results showed that there were obvious differences in the microstructure of trabeculae in the early stage of femoral head necrosis in emus. The results of 4.RT-PCR showed that the expression of bone resorption related genes RANKL and RANK was higher in bone destruction area than in hyperplastic bone area and normal area. The expression of bone formation related genes BMP2 and OPG in the hyperosteogenic and normal areas was higher than that in the bone destruction area. Conclusion: 1. The model of femoral head necrosis in emus can be established by injecting anhydrous ethanol. 2. The osteonecrosis of femoral head in emus showed obvious zoning difference. The density of trabecular bone became smaller and the density of trabecular bone increased in the area of bone destruction. The activity of osteoblasts and osteoclasts in osteoblasts were increased.
【学位授予单位】：石河子大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R681.8
本文编号：2349445
[Abstract]:Objective: to establish an emu model of femoral head necrosis which can mimic the early manifestation of human femoral head necrosis, to study the characteristics of bone microstructure in different areas of femoral head necrosis in emus, and to explore the causes of femoral head necrosis. In order to seek a safe and effective clinical head-keeping treatment. Methods: adult emus were used to establish the animal model of femoral head osteonecrosis by local injection of anhydrous alcohol. The femoral head specimens were examined by Micro-CT and pathology. According to the difference of bone density, each specimen was divided into bone destruction area, bone hyperplasia area and normal area. The microstructure of bone in different areas was observed and compared by histological examination and Micro-CT examination. The activity of osteoclasts in different regions was observed and analyzed by real-time fluorescence quantitative PCR. Results: 1. The osteonecrosis model of femoral head of emus was established by local injection of anhydrous ethanol. There was no collapse of the femoral head and various degrees of osteonecrosis appeared in the femoral head. The bone metrology results showed that the trabecular thickness (Tb.th) of the model side increased by 87% and the trabecular spacing (Tb.sp) decreased by 43% compared with the control side. There was no significant change in bone mineralization density (BMD) and bone trabecular number (Tb.N). 2.Micro-CT analysis showed that there were significant regional differences in femoral head necrosis specimens. The bone trabeculae in the bone destruction area appeared fracture, the continuity destruction; the bone trabecular space became narrow, the number increased in the hyperosteogenic area; the normal area bone trabecular thickness distribution is uniform, the structure is complete. The BMD,Tb.Th,Tb.N of trabecular bone in bone destruction area was lower than that in normal area, and Tb.Sp was higher than that in normal area (P0.05). The BMD,Tb.Th,Tb.N of bone trabeculae in hypertrophic area was higher than that in normal area, and Tb.Sp was lower than that in normal area (P0.05). The pathological results showed that there were obvious differences in the microstructure of trabeculae in the early stage of femoral head necrosis in emus. The results of 4.RT-PCR showed that the expression of bone resorption related genes RANKL and RANK was higher in bone destruction area than in hyperplastic bone area and normal area. The expression of bone formation related genes BMP2 and OPG in the hyperosteogenic and normal areas was higher than that in the bone destruction area. Conclusion: 1. The model of femoral head necrosis in emus can be established by injecting anhydrous ethanol. 2. The osteonecrosis of femoral head in emus showed obvious zoning difference. The density of trabecular bone became smaller and the density of trabecular bone increased in the area of bone destruction. The activity of osteoblasts and osteoclasts in osteoblasts were increased.
【学位授予单位】：石河子大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R681.8
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