微流控芯片流体剪切力和肿瘤坏死因子-α共同作用对大鼠软骨细胞表型的影响

发布时间：2018-12-08 14:11

【摘要】：流体剪切力是生物体内普遍存在的一种生物力学形式,是细胞微环境的重要组成部分,对细胞多种生物学行为有重要调节作用。该研究以微流控芯片技术为基础,建立了一种基于流阻原理能同时产生4个不同大小流体剪切力的微流控芯片平台,用以研究低流速的流体剪切力对大鼠原代软骨细胞表型维持的影响。结果表明,流体剪切力可促进软骨细胞的表型维持。还加入了肿瘤坏死因子-α(TNF-α),考察流体剪切力和TNF-α共同作用对软骨细胞表型的影响。结果表明,在剪切力和TNF-α共同作用下,软骨细胞的Ⅱ型胶原和蛋白多糖表达明显下调。该研究为软骨组织工程和骨性关节炎的疾病研究提供有力的研究平台,为骨关节疾病治疗和防治提供了理论依据。
[Abstract]:Fluid shear stress is a common biomechanical form in organisms and an important component of cell microenvironment. It plays an important role in regulating many biological behaviors of cells. Based on the technology of microfluidic chip, a microfluidic chip platform based on the principle of flow resistance can generate four different fluid shear forces at the same time. To study the effect of low velocity fluid shear stress on phenotypic maintenance of rat primary chondrocytes. The results showed that fluid shear stress promoted phenotypic maintenance of chondrocytes. Tumor necrosis factor- 伪 (TNF- 伪) was added to investigate the effects of fluid shear stress and TNF- 伪 on the phenotype of chondrocytes. The results showed that the expression of type 鈪，

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