PDO-SIS补片的生物力学性能及其对大鼠腹壁缺损的修复作用

发布时间：2018-12-14 22:01

【摘要】：目的探讨采用猪脱细胞小肠黏膜下组织(P-SIS)和聚二恶烷酮(PDO)合成的一种杂化补片——PDO-SIS补片的力学性能及其对大鼠腹壁部分缺损的修复作用。方法通过编织、真空层压等步骤构建PDO-SIS补片和8SIS补片,并测量其相关力学性能。取健康雌性SD大鼠36只,在腹部两侧各制作一个1cm×1cm的腹壁部分缺损,分别以等面积的PDO-SIS补片、8SIS补片进行修复,分别于术后2、4、8周随机选取12只大鼠取材并进行评估。结果 PDO-SIS补片的弯曲长度和拉断力均大于8SIS补片(P0.05),且均随PDO材料直径的增加而增加。术后各时间点两组均未观察到急性炎症反应。术后2周时,PDO-SIS补片组和8SIS补片组中均可观察到中等程度的慢性炎症反应,但随时间延长炎症反应逐渐减轻,至术后8周时两组均未见明显的慢性炎症反应,组间差异无统计学意义(P0.05)。两种补片材料在修复区内均逐渐降解,再生的胶原组织不断沉积,两组在胶原含量和排列方面均无明显差异(P0.05)。结论 PDO-SIS补片与8SIS补片相比具有更好的力学性能,在修复大鼠腹壁部分缺损时不引起强烈的免疫排斥反应。
[Abstract]:Objective to investigate the mechanical properties of porcine acellular small intestinal submucosal tissue (P-SIS) and polydioxanone (PDO) hybrid patch, PDO-SIS patch, and its effect on the repair of partial abdominal wall defects in rats. Methods PDO-SIS patch and 8SIS patch were constructed by braiding and vacuum lamination, and their mechanical properties were measured. A partial defect of abdominal wall of 1cm 脳 1cm was made on each side of abdomen of 36 healthy female SD rats. The same area of PDO-SIS patch and 8SIS patch were used to repair the defect. Twelve rats were randomly selected and evaluated at 2 weeks and 48 weeks after operation. Results the bending length and tensile force of PDO-SIS patch were larger than that of 8SIS patch (P0.05), and both increased with the increase of PDO material diameter. No acute inflammatory reaction was observed in both groups at each time point after operation. At 2 weeks after operation, moderate chronic inflammatory reaction was observed in PDO-SIS supplement group and 8SIS supplement group, but gradually decreased with time, and no obvious chronic inflammatory reaction was observed in both groups at 8 weeks after operation. There was no significant difference between groups (P0.05). The two kinds of patch materials were gradually degraded and regenerated collagen tissue was continuously deposited in the repair area. There was no significant difference in collagen content and arrangement between the two groups (P0.05). Conclusion compared with 8SIS patch, PDO-SIS patch has better mechanical properties and does not cause strong immune rejection in repairing partial defect of abdominal wall in rats.
【作者单位】：第二军医大学长征医院普外一科;上海市静安区闸北中心医院检验科;
【基金】：国家自然科学基金(81471859) 上海市卫计委科研基金(201540388)~~
【分类号】：R656.3

【相似文献】