阿司匹林对周围神经损伤修复影响的实验性研究
发布时间：2018-12-19 18:23
【摘要】：目的:本实验旨在探寻阿司匹林是否对周围神经损伤恢复具有促进作用。方法:雄性SD大鼠30只,体重在200-220克,随机分为阿司匹林组及生理盐水组。所有大鼠左侧坐骨神经完全离断,10倍显微镜下行神经吻接术,实验组给予阿司匹林溶液(10mg/ml、50mg/kg)灌胃,1次/日,对照组给予等量生理盐水灌胃。术后观察大鼠大体精神状态、饮食情况及展爪反射等指标;术后第2、4、6、8周分别计算两组大鼠坐骨神经功能指数(SFI);术后第2、4、6周分别测量感觉神经再生距离(Pinch test);术后第2、4、6、8周分别取大鼠双侧腓肠肌,称重并计算肌肉湿重比;术后第6、8周分别取腓肠肌固定、染色,Olympus软件下测量直径;术后第8周测量大鼠坐骨神经传导速率、潜伏期、波幅、并计算传导速度恢复率;术后第4、8周分别取大鼠左侧坐骨神经行免疫组化S-100抗体染色,应用Olympus软件并计算单位视野中雪旺细胞的数量并进行统计学分析;术后第8周电镜下观察雪旺细胞增生情况及神经髓鞘排列变化情况。结果:大体观察:阿司匹林组的精神状态、饮食状况相比于对照组更佳。阿司匹林组大鼠展爪反射、分趾功能恢复较早,约在术后3~4周,对照组展爪反射出现较晚,约术后第6周有所改善;术后第4周可见生理盐水组神经吻合口远端神经较近端变细,神经弹性减弱,吻合口未见明显血管通过,阿司匹林组可见神经吻合口有少量血管通过,神经弹性良好,远端神经较近端神经直径并无明显差异;坐骨神经功能指数(SFI):术后2周,因大鼠术侧足趾挛缩,自然下垂,足爪不能展开,无法支撑着地,坐骨功能指数不能检测。术后第4、6、8周阿司匹林修复组坐骨神经指数优于生理盐水组,差异具有统计学意义(P0.05);感觉神经再生距离(Pinch test):阿司匹林组较生理盐水组恢复距离更长,差别具有统计学意义(P0.05);腓肠肌肌湿重比恢复率除第2周无统计学差异外,4、6、8周阿司匹林组均优于对照组,差异具有统计学意义(P0.05);术后6、8周腓肠肌直径阿司匹林组均大于同期生理盐水对照组,差异具有统计学差异(P0.05);神经电生理检测:阿司匹林组潜伏期低于对照组,差异具有统计学意义(P0.05),阿司匹林组波幅高于对照组,差别具有统计学意义(P0.05),实验组传导速度快于对照组,差异具有统计学差异(P0.05);免疫组织化学:术后第4、8周阿司匹林组S-100蛋白表达及分布情况均优于同时期生理盐水组,实验组较对照组雪旺细胞增生明显且具有统计学意义(P0.05);电镜显示:阿司匹林组神经髓鞘厚度高、外形规则呈圆形或椭圆形,阿司匹林组具有完整鞘膜的神经纤维,鞘膜增厚,排列较整齐,阿司匹林组髓鞘板层分布较好,厚度较均匀,排列整齐,对照组神经纤维板层略疏松,髓鞘扭曲明显,厚度不一,形状不规则。结论:阿司匹林具有促进周围神经损伤修复作用
[Abstract]:Objective: to explore whether aspirin can promote the recovery of peripheral nerve injury. Methods: thirty male SD rats, weighing 200-220 g, were randomly divided into aspirin group and saline group. The left sciatic nerve of all rats was completely disconnected, and the nerve anastomosis was performed under 10 times microscope. The experimental group was given aspirin solution (10 mg / ml 50 mg / kg) once a day, and the control group was given the same amount of normal saline. After operation, the general mental state, diet and paw reflex of rats were observed. The sciatic nerve function index (SFI);) of the two groups was calculated at 6 weeks after operation, and the sensory nerve regeneration distance (Pinch test);) was measured at 6 weeks after operation. The bilateral gastrocnemius muscles were taken from rats at the 2nd week and 4th week after operation, and the muscle wet weight ratio was calculated, and the gastrocnemius muscle was fixed, stained, and the diameter was measured by Olympus software at the 8th week after the operation, the gastrocnemius muscle was fixed, stained, and the diameter was measured by Olympus software. The conduction rate, latency and amplitude of sciatic nerve were measured at the 8th week after operation, and the recovery rate of conduction velocity was calculated. The left sciatic nerve of the rats was harvested for S-100 antibody staining at the 4th week after operation. The number of Schwann cells in the unit field was calculated by Olympus software and analyzed statistically. Schwann cell proliferation and myelin sheath arrangement were observed under electron microscope 8 weeks after operation. Results: general observation: the mental state and diet of aspirin group were better than that of control group. The paw extension reflex of the aspirin group recovered earlier than that of the control group at 3 ~ 4 weeks postoperatively, and the paw extension reflex in the control group was later than that in the control group, and was improved at the 6th week after operation. At the 4th week after operation, the distal nerve of the nerve anastomosis in the saline group became thinner, the nerve elasticity decreased, and no obvious blood vessels passed through the anastomotic stoma. In the aspirin group, a small amount of blood vessels were seen passing through the nerve anastomotic stoma, and the nerve elasticity was good. There was no significant difference in the diameter of the distal nerve compared with the proximal nerve. The sciatic nerve function index (SFI):) 2 weeks after operation, due to the contracture of the toes of the surgical side of rats, natural prolapse, feet can not be expanded, unable to support the ground, the ischium function index can not be measured. The sciatic nerve index of aspirin repair group was better than that of normal saline group at the 4th week after operation (P0.05). The distance of sensory nerve regeneration in (Pinch test): aspirin group was longer than that in normal saline group, the difference was statistically significant (P0.05). The recovery rate of wet weight ratio of gastrocnemius muscle was significantly higher than that of control group (P0.05), except that there was no statistical difference in the recovery rate of gastrocnemius muscle wet weight ratio at the 2nd week, the aspirin group at the 4th week was better than the control group (P0.05). The diameter of gastrocnemius muscle in aspirin group was higher than that in saline group at 6 and 8 weeks postoperatively (P0.05). Electrophysiological examination: the latency of aspirin group was lower than that of control group, the difference was statistically significant (P0.05), the amplitude of wave in aspirin group was higher than that in control group, the difference was statistically significant (P0.05). The conduction velocity of the experimental group was faster than that of the control group, and the difference was statistically significant (P0.05). Immunohistochemistry: the expression and distribution of S-100 protein in aspirin group was better than that in saline group at the 4th week after operation, and the proliferation of Schwann cells in experimental group was significantly higher than that in control group (P0.05). Electron microscope showed that the thickness of nerve myelin sheath in aspirin group was high, and the shape of nerve myelin sheath was round or ellipse. In aspirin group, the nerve fibers of intact sheath were thickened and arranged neatly, and the myelin sheathing lamina of aspirin group was well distributed. In the control group, the neurofiberboard layer was looser, the myelin sheath was distorted obviously, the thickness was different and the shape was irregular. Conclusion: aspirin can promote the repair of peripheral nerve injury.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R651.3
本文编号：2387286
[Abstract]:Objective: to explore whether aspirin can promote the recovery of peripheral nerve injury. Methods: thirty male SD rats, weighing 200-220 g, were randomly divided into aspirin group and saline group. The left sciatic nerve of all rats was completely disconnected, and the nerve anastomosis was performed under 10 times microscope. The experimental group was given aspirin solution (10 mg / ml 50 mg / kg) once a day, and the control group was given the same amount of normal saline. After operation, the general mental state, diet and paw reflex of rats were observed. The sciatic nerve function index (SFI);) of the two groups was calculated at 6 weeks after operation, and the sensory nerve regeneration distance (Pinch test);) was measured at 6 weeks after operation. The bilateral gastrocnemius muscles were taken from rats at the 2nd week and 4th week after operation, and the muscle wet weight ratio was calculated, and the gastrocnemius muscle was fixed, stained, and the diameter was measured by Olympus software at the 8th week after the operation, the gastrocnemius muscle was fixed, stained, and the diameter was measured by Olympus software. The conduction rate, latency and amplitude of sciatic nerve were measured at the 8th week after operation, and the recovery rate of conduction velocity was calculated. The left sciatic nerve of the rats was harvested for S-100 antibody staining at the 4th week after operation. The number of Schwann cells in the unit field was calculated by Olympus software and analyzed statistically. Schwann cell proliferation and myelin sheath arrangement were observed under electron microscope 8 weeks after operation. Results: general observation: the mental state and diet of aspirin group were better than that of control group. The paw extension reflex of the aspirin group recovered earlier than that of the control group at 3 ~ 4 weeks postoperatively, and the paw extension reflex in the control group was later than that in the control group, and was improved at the 6th week after operation. At the 4th week after operation, the distal nerve of the nerve anastomosis in the saline group became thinner, the nerve elasticity decreased, and no obvious blood vessels passed through the anastomotic stoma. In the aspirin group, a small amount of blood vessels were seen passing through the nerve anastomotic stoma, and the nerve elasticity was good. There was no significant difference in the diameter of the distal nerve compared with the proximal nerve. The sciatic nerve function index (SFI):) 2 weeks after operation, due to the contracture of the toes of the surgical side of rats, natural prolapse, feet can not be expanded, unable to support the ground, the ischium function index can not be measured. The sciatic nerve index of aspirin repair group was better than that of normal saline group at the 4th week after operation (P0.05). The distance of sensory nerve regeneration in (Pinch test): aspirin group was longer than that in normal saline group, the difference was statistically significant (P0.05). The recovery rate of wet weight ratio of gastrocnemius muscle was significantly higher than that of control group (P0.05), except that there was no statistical difference in the recovery rate of gastrocnemius muscle wet weight ratio at the 2nd week, the aspirin group at the 4th week was better than the control group (P0.05). The diameter of gastrocnemius muscle in aspirin group was higher than that in saline group at 6 and 8 weeks postoperatively (P0.05). Electrophysiological examination: the latency of aspirin group was lower than that of control group, the difference was statistically significant (P0.05), the amplitude of wave in aspirin group was higher than that in control group, the difference was statistically significant (P0.05). The conduction velocity of the experimental group was faster than that of the control group, and the difference was statistically significant (P0.05). Immunohistochemistry: the expression and distribution of S-100 protein in aspirin group was better than that in saline group at the 4th week after operation, and the proliferation of Schwann cells in experimental group was significantly higher than that in control group (P0.05). Electron microscope showed that the thickness of nerve myelin sheath in aspirin group was high, and the shape of nerve myelin sheath was round or ellipse. In aspirin group, the nerve fibers of intact sheath were thickened and arranged neatly, and the myelin sheathing lamina of aspirin group was well distributed. In the control group, the neurofiberboard layer was looser, the myelin sheath was distorted obviously, the thickness was different and the shape was irregular. Conclusion: aspirin can promote the repair of peripheral nerve injury.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R651.3
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