右美托咪定对重度颅脑创伤大鼠脑组织AQP4表达的影响

发布时间：2018-12-26 13:30

【摘要】：[目的] 研究右美托咪定对重度颅脑创伤后大鼠脑水肿区组织AQP-4表达的影响及脑水肿变化情况。[材料与方法] 选用健康成年SD大鼠180只,体重为220-250g,根据随机化原则分为假手术组(对照组,n=60)；颅脑创伤模型组(模型组,n=60)；颅脑创伤右美托咪啶治疗组(治疗组,n=60)。参照Feeney自由落体颅脑损伤模型制作方法制做重度颅脑创伤模型,右美托咪定治疗组造模成功后即刻经鼠尾静脉持续泵入右美托咪定,假手术组和TBI模型组持续泵入相同剂量0.9%氯化钠注射液,于创伤后6h,24h,48h,72h进行神经损害程度评分(Neurological Severity Score, NSS)后断头取脑。用干/湿比重法和伊文思蓝测定法观察大鼠TBI后不同时间脑组织含水量和BBB通透性的变化,通过HE染色方法观察脑组织形态学变化；采用western blot法检测脑水肿区AQP4表达情况。[结果] 模型组和治疗组NSS评分、脑组织含水量、伊文思蓝含量和水肿区AQP4表达均高于对照组(P0.05)；模型组大鼠NSS评分,脑组织含水量,伊文思蓝含量和水肿区AQP4表达量均比治疗组高(P0.05)；模型组和对照组TBI后6h NSS评分、脑组织含水量、EB含量及水肿区AQP4表达水平均增高,24h达高峰,24h后逐渐减轻,但直至72h时仍较对照组高(P0.05)。[结论] 结果表明局灶性脑损伤后AQP-4蛋白的表达与EB含量、脑组织含水量及NSS评分呈正相关。TBI大鼠脑水肿高峰为TBI后24h；脑水肿严重程度与脑水肿区AQP4表达、BBB通透性呈正相关,随着AQP4表达减少,BBB通透性降低,脑水肿逐渐减轻。TBI大鼠使用右美托咪定镇静治疗在损伤早期(24h内)即可以抑制AQP4蛋白的表达,降低BBB通透性及减轻大鼠脑水肿的程度。
[Abstract]:[objective] to study the effect of dexmetomidine on the expression of AQP-4 and the changes of brain edema in rats with severe traumatic brain injury. [materials and methods] 180 healthy adult SD rats, weighing 220 to 250 g, were randomly divided into sham-operation group (control group, n = 60), craniocerebral trauma model group (model group, n = 60), and control group (n = 60). Dexmetidine treatment group for traumatic brain injury (treatment group, n = 60). According to the model of Feeney free falling body craniocerebral injury, the model of severe craniocerebral injury was made. After the successful establishment of the model, dexmetomidine was continuously pumped into the rat tail vein immediately after the treatment group was treated with dexmetomidine. The sham operation group and the TBI model group were continuously pumped with 0.9% sodium chloride injection at the same dose. The brain was taken from the head of the sham operation group and the TBI model group at 24 hours, 48 hours and 72 hours after the injury. (Neurological Severity Score, NSS) was used to evaluate the degree of nerve damage. The changes of brain water content and BBB permeability at different time after TBI were observed by dry / wet specific gravity method and Evans blue method. The morphological changes of brain tissue were observed by HE staining, and the expression of AQP4 in brain edema area was detected by western blot method. [results] the NSS score, water content of brain tissue, Evans blue content and AQP4 expression in edema area in model group and treatment group were higher than those in control group (P0.05). The NSS score, water content of brain tissue, Evans blue content and AQP4 expression in edema area in the model group were higher than those in the treatment group (P0.05). The NSS score, brain water content, EB content and AQP4 expression in edema area increased in model group and control group at 6 h after TBI, reached the peak at 24 h, gradually decreased after 24 h, but were still higher than that in control group at 72 h (P0.05). [conclusion] the results showed that the expression of AQP-4 protein was positively correlated with EB content, brain water content and NSS score after focal brain injury. The peak of brain edema in TBI rats was 24 hours after TBI. The severity of cerebral edema was positively correlated with the expression of AQP4 and the permeability of BBB in the brain edema area. With the decrease of AQP4 expression, the permeability of BBB decreased. TBI rats treated with dexmetidine could inhibit the expression of AQP4 protein, reduce the permeability of BBB and reduce the degree of brain edema in the early stage of injury (within 24 hours).
【学位授予单位】：昆明医科大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R614

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