Ski在大鼠活化星形胶质细胞中表达的变化

发布时间：2018-12-27 14:49

【摘要】：目的:探究Ski在大鼠正常及活化星形胶质细胞中的表达及随时间变化的规律。方法:提取大鼠大脑皮质,分离星形胶质细胞,体外培养。采用LPS刺激和细胞划痕损伤2种方法活化星形胶质细胞,均采用Western blot法检测各个时点2种活化星形胶质细胞中Ski和胶质纤维酸性蛋白(GFAP)蛋白表达情况,利用间接免疫荧光法检测Ski蛋白在星形胶质细胞中的表达定位。结果:GFAP蛋白在星形胶质细胞中固有表达,LPS活化和划痕损伤后表达均上调。Ski在正常星形胶质细胞中呈低表达状态,1 mg/L LPS活化星形胶质细胞后,Ski表达开始增加,4 d时表达量最高(P0.05),6 d时开始下降,但仍高于未活化组;细胞划痕损伤后Ski蛋白表达情况与上述情况高度一致。Ski在正常及LPS活化6 d星形胶质细胞中表达主要集中在胞核,LPS活化后2和4 d时在胞质中出现明确的Ski表达。结论:Ski蛋白表达于星形胶质细胞,并在活化星形胶质细胞中表达上调。由此,我们推测Ski蛋白可能调控星形胶质细胞的活化、增殖等过程。
[Abstract]:Aim: to investigate the expression and changes of Ski in normal and activated astrocytes of rats. Methods: astrocytes were isolated from rat cerebral cortex and cultured in vitro. The expression of Ski and glial fibrillary acidic protein (GFAP) (GFAP) in the activated astrocytes were detected by Western blot method at each time point, and the activation of astrocytes was induced by LPS stimulation and cell scratch injury, and the expression of Ski and glial fibrillary acidic protein (GFAP) (GFAP) protein were detected by Western blot method. The expression of Ski protein in astrocytes was detected by indirect immunofluorescence assay. Results: the intrinsic expression of GFAP protein in astrocytes was up-regulated by LPS activation and scratch injury. The expression of Ski was low in normal astrocytes, and the expression of Ski began to increase after 1 mg/L LPS activation of astrocytes. The expression level was the highest at the 4th day (P0.05), and decreased at the 6th day, but still higher than that in the non-activated group. The expression of Ski protein in normal and LPS activated astrocytes was mainly concentrated in the nucleus of the astrocytes after scratch injury, and Ski was expressed in the cytoplasm at 2 and 4 days after LPS activation. Conclusion: Ski protein is expressed in astrocytes and up-regulated in activated astrocytes. Therefore, we speculate that Ski protein may regulate the activation and proliferation of astrocytes.
【作者单位】：兰州大学第二医院骨科;兰州大学第二医院甘肃省骨关节疾病研究重点实验室;第三军医大学大坪医院野战外科研究所三室创伤烧伤与复合伤国家重点实验室;
【基金】：国家自然科学基金资助项目(No.30772299)
【分类号】：R651.2

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