骨髓间充质干细胞在肝细胞损伤恢复中的作用
发布时间：2018-12-31 21:05
【摘要】：目的:研究骨髓间充质干细胞(BMSCs)在肝细胞损伤恢复中的作用,并探讨可能机制。方法:(1)培养原代肝细胞及BMSCs并鉴定;(2)建立损伤肝细胞模型;(3)MTT法检测不同条件下肝细胞增殖情况;(4)加入BMSCs、bax抑制剂、BMSCs+bax抑制剂进行共培养,采用Western-blot和real-time PCR法检测TGF-β1、bcl-2、bax等指标。结果:(1)BMSCs+HGF共培养组中的OD值明显高于BMSCs或HGF单独联合培养组(P0.05),BMSCs与HGF单独共培养组之间OD值差异无统计学意义,而它们与对照组相比差异均有统计学意义(P0.05)。(2)bax1抑制剂共培养组与BMSCs共培养组的OD值差异无统计学意义,两者与对照组相比差异有统计学意义(P0.05),BMSCs+Bax-1共培养组的OD值与BMSCs或bax1共培养组差异无统计学意义。(3)Western-blot法及real-time PCR检测结果提示TGF-β1、bax在bax1抑制剂共培养组与BMSCs共培养组中均显著低于对照组(P0.05),而BMSCs+Bax-1抑制剂共培养组与两者单独共培养差异无统计学意义,Bcl-2结果与前两者相反。结论:BMSCs在肝细胞损伤恢复中能促进肝细胞增殖,BMSCs促进肝细胞增殖通过调节TGF-β1/bcl-2(bax)这个信号通道减少凋亡来实现的。
[Abstract]:Aim: to study the role of bone marrow mesenchymal stem cell (BMSCs) in the recovery of hepatocyte injury and explore the possible mechanism. Methods: (1) primary hepatocytes and BMSCs were cultured and identified; (2) hepatocyte injury model was established; (3) MTT method was used to detect the proliferation of hepatocytes under different conditions. (4) BMSCs,bax inhibitor, BMSCs bax inhibitor was added to co-culture, Western-blot and real-time PCR methods were used to detect TGF- 尾 1 bcl-2bax and so on. Results: (1) the OD value in) BMSCs HGF co-culture group was significantly higher than that in BMSCs or HGF co-culture group (P0.05). There was no significant difference in OD value between), BMSCs and HGF co-culture group. There was no significant difference in OD between bax1 inhibitor co-culture group and BMSCs co-culture group (P0.05), but there was significant difference between them compared with control group (P0.05). The OD value of BMSCs Bax-1 coculture group was not significantly different from that of BMSCs or bax1 co-culture group. (3) the results of Western-blot and real-time PCR showed that TGF- 尾 _ 1 was not significantly different from that of BMSCs or bax1 co-culture group. Bax was significantly lower in bax1 inhibitor co-culture group and BMSCs co-culture group than in control group (P0.05), but there was no significant difference between BMSCs Bax-1 inhibitor co-culture group and BMSCs Bax-1 inhibitor co-culture group. Bcl-2 results were contrary to the former two groups. Conclusion: BMSCs can promote the proliferation of hepatocytes during the recovery of hepatocyte injury, and BMSCs can promote the proliferation of hepatocytes by regulating the signal channel of TGF- 尾 1/bcl-2 (bax) to reduce apoptosis.
【作者单位】： 解放军第16医院普外科;解放军第16医院医务处;第三军医大学西南医院全军肝胆外科研究所;
【基金】：全军医学科技青年培育项目(编号:13QNP023)
【分类号】：R657.3
本文编号：2397111
[Abstract]:Aim: to study the role of bone marrow mesenchymal stem cell (BMSCs) in the recovery of hepatocyte injury and explore the possible mechanism. Methods: (1) primary hepatocytes and BMSCs were cultured and identified; (2) hepatocyte injury model was established; (3) MTT method was used to detect the proliferation of hepatocytes under different conditions. (4) BMSCs,bax inhibitor, BMSCs bax inhibitor was added to co-culture, Western-blot and real-time PCR methods were used to detect TGF- 尾 1 bcl-2bax and so on. Results: (1) the OD value in) BMSCs HGF co-culture group was significantly higher than that in BMSCs or HGF co-culture group (P0.05). There was no significant difference in OD value between), BMSCs and HGF co-culture group. There was no significant difference in OD between bax1 inhibitor co-culture group and BMSCs co-culture group (P0.05), but there was significant difference between them compared with control group (P0.05). The OD value of BMSCs Bax-1 coculture group was not significantly different from that of BMSCs or bax1 co-culture group. (3) the results of Western-blot and real-time PCR showed that TGF- 尾 _ 1 was not significantly different from that of BMSCs or bax1 co-culture group. Bax was significantly lower in bax1 inhibitor co-culture group and BMSCs co-culture group than in control group (P0.05), but there was no significant difference between BMSCs Bax-1 inhibitor co-culture group and BMSCs Bax-1 inhibitor co-culture group. Bcl-2 results were contrary to the former two groups. Conclusion: BMSCs can promote the proliferation of hepatocytes during the recovery of hepatocyte injury, and BMSCs can promote the proliferation of hepatocytes by regulating the signal channel of TGF- 尾 1/bcl-2 (bax) to reduce apoptosis.
【作者单位】： 解放军第16医院普外科;解放军第16医院医务处;第三军医大学西南医院全军肝胆外科研究所;
【基金】：全军医学科技青年培育项目(编号:13QNP023)
【分类号】：R657.3
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