组织工程窦房结构建及血管化的最新研究与应用进展

发布时间：2019-01-05 00:23

【摘要】：背景:理论上组织工程窦房结移植入体内可以建立新的起搏点,并且可以随着机体发育,达到治疗窦房结综合征的目的,但目前仍处于最初级的阶段,需要进行不断的探索。目的:总结综述现阶段组织工程窦房结构建的不同方法及心脏组织工程血管化的研究进展。方法:由第一作者检索Pub Med数据库及CNKI全文数据库1984年至2016年的相关文献,并进行筛选,归纳和总结。英文检索词为"Vascularize,Tissue Engineering,Sinus Node",中文检索词为"血管化、组织工程、窦房结"。阐述组织工程窦房结的构建方法,总结归纳了各种心脏组织工程血管化的重要性及策略,分析组织工程窦房结血管化应用的方法,最后组织工程窦房结血管化进行总结和展望。结果与结论:起搏种子细胞的选择大致为窦房结原代细胞,脂肪、骨髓间充质干细胞,胚胎干细胞等。组织工程支架材料更是种类繁多,活体的窦房结本身就处于胶原支架上,因此胶原类材料更为适用于组织工程窦房结。3D打印技术、细胞膜片技术使血管化组织工程窦房结的构建和移植成为可能。移植早期的血液供应,是窦房结移植是否成功的关键,组织工程窦房结血管化的相关文献在仍处于空白状态,内皮祖细胞作为成血管能力较强的细胞已经有了巨大的进展,但是仍有一些缺陷,还面临着重大的挑战。
[Abstract]:Background: in theory, tissue engineering sinus node transplantation into the body can establish a new pacing point, and with the development of the body, can achieve the purpose of treating sinus node syndrome, but at present, it is still in the most primary stage, and need to be continuously explored. Objective: to summarize the different methods of tissue engineering sinus node construction and the research progress of heart tissue engineering vascularization. Methods: Pub Med database and CNKI full text database were searched by the first author from 1984 to 2016. The key words were "Vascularize,Tissue Engineering,Sinus Node" in English and "vascularization, tissue engineering, sinus node" in Chinese. This paper expounds the construction methods of tissue engineering sinus node, summarizes the importance and strategy of various kinds of heart tissue engineering vascularization, analyzes the application methods of tissue engineering sinus node vascularization, and finally summarizes and prospects the vascularization of tissue engineering sinus node. Results and conclusion: the selection of pacemaker seed cells was mainly sinoatrial node primary cells, fat, bone marrow mesenchymal stem cells, embryonic stem cells and so on. Tissue engineering scaffolds have a wide variety of materials, and the living sinoatrial node itself is on the collagen scaffold, so collagen materials are more suitable for tissue engineering sinus node. 3D printing technology. Membrane patch technique makes it possible to construct and transplant the vascularized tissue engineered sinus node. The blood supply in the early stage of transplantation is the key to the success of sinoatrial node transplantation. The literature on vascularization of tissue engineering sinus node is still blank, and endothelial progenitor cells have made great progress as vascular forming cells. But there are still some flaws and major challenges.
【作者单位】：滨州医学院烟台校区;解放军第二军医大学;
【基金】：国家自然科学基金(81071603,30970737) 上海市自然科学重点基金(09JC1417700)~~
【分类号】：R318.08;R654

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