RCAN1及CnA在下肢闭塞性动脉硬化支架术后再狭窄组织中的表达及意义

发布时间：2019-01-07 18:19

【摘要】：目的·观察钙调神经磷酸酶调节因子1(RCAN1)、钙调神经磷酸酶A(Cn A)在下肢闭塞性动脉硬化介入术后支架内再狭窄组织中的表达,探讨其与支架内再狭窄的相关性。方法·收集2013年9月至2016年6月重庆医科大学附属第一医院血管外科中心下肢闭塞性动脉硬化股浅动脉支架内再狭窄血管标本15例,取支架狭窄段、支架近端、支架远端血管组织,采用H-E及Masson染色观察组织形态学变化;Western blotting检测RCAN1、Cn A、增殖细胞核抗原(PCNA)的表达;免疫组化及免疫荧光检测RCAN1、Cn A蛋白的表达及分布;免疫共沉淀检测RCAN1蛋白和Cn A蛋白的相互作用。结果·RCAN1在支架远端组织的表达水平显著高于在支架近端组织和支架狭窄段组织的表达水平(P0.05),RCAN1在支架近端组织的表达水平显著高于在支架狭窄段组织的表达水平(P0.05);Cn A和PCNA在支架狭窄段组织的表达水平显著高于在支架近端组织、支架远端组织的表达水平(P0.05);免疫组化及免疫荧光染色结果显示RCAN1和Cn A主要在血管平滑肌细胞质中表达;免疫共沉淀结果显示RCAN1与Cn A在血管组织中存在相互作用。结论·RCAN1的低水平表达与Cn A的表达水平增高可能与支架内再狭窄发生有关。
[Abstract]:Objective to investigate the expression of calmodulin neurophosphatase 1 (RCAN1) and calmodulin A (Cn A) in restenosis of stents after lower extremity occlusive arteriosclerosis intervention. Methods from September 2013 to June 2016, 15 cases of restenosis were collected from vascular surgery center of the first affiliated Hospital of Chongqing Medical University. Histomorphologic changes were observed by H-E and Masson staining. Western blotting was used to detect the expression of RCAN1,Cn A and proliferating cell nuclear antigen (PCNA), immunohistochemistry and immunofluorescence were used to detect the expression and distribution of RCAN1,Cn A protein, and the interaction between RCAN1 protein and Cn A protein was detected by immunoprecipitation. Results the expression level of RCAN1 in distal stents was significantly higher than that in proximal stents and stenosed stents (P0.05). The expression level of RCAN1 in proximal stents was significantly higher than that in stenosed stents (P0.05). The expression levels of Cn A and PCNA in the stenosed stents were significantly higher than those in the proximal stents and distal stents (P0.05). The results of immunohistochemistry and immunofluorescence staining showed that RCAN1 and Cn A were mainly expressed in the cytoplasm of vascular smooth muscle, and the results of co-immunoprecipitation showed that there was interaction between RCAN1 and Cn A in vascular tissue. Conclusion the low expression of RCAN1 and the increase of Cn A expression may be associated with restenosis in stent.
【作者单位】：重庆医科大学附属第一医院血管外科;
【基金】：国家临床重点专科建设项目(卫办医政函【2012】649)~~
【分类号】：R654.4

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