重复经颅磁刺激联合嗅鞘细胞移植治疗脊髓损伤

发布时间：2019-01-22 10:46

【摘要】：背景:传统观念认为哺乳动物的脑和脊髓损伤后不能再生,脊髓损伤后功能恢复的预后希望很渺茫。目的:分析经颅磁刺激联合嗅鞘细胞移植治疗大鼠脊髓损伤的修复效果。方法:对80只大鼠进行脊髓损伤模型制备,随机等分成4组,胸髓横断损伤组、嗅鞘细胞移植组、重复经颅磁刺激组及联合组。各组大鼠行T10全椎板切除建立胸髓横断损伤动物模型,造模24 h后,嗅鞘细胞移植组和联合组大鼠在损伤区附近注射嗅鞘细胞,重复经颅磁刺激组及联合组大鼠于左前囟上方接受重复经颅磁刺激治疗。结果与结论:移植4周后,与其他3组相比,重复经颅磁刺激联合嗅鞘细胞移植组大鼠运动功能恢复明显,运动功能BBB评分升高,坐骨神经电生理功能明显恢复,损伤脊髓修复较好。提示重复经颅磁刺激联合嗅鞘细胞移植治疗大鼠脊髓损伤可以获得显著疗效,两者联合应用可明显促进脊髓损伤后的轴突再生及功能恢复。
[Abstract]:Background: the traditional view is that mammalian brain and spinal cord injury can not regenerate, and the prognosis of functional recovery after spinal cord injury is very poor. Objective: to analyze the effect of transcranial magnetic stimulation combined with olfactory ensheathing cell transplantation in the treatment of spinal cord injury in rats. Methods: 80 rats were randomly divided into 4 groups: thoracic spinal cord injury group, olfactory ensheathing cell transplantation group, repetitive transcranial magnetic stimulation group and combined group. Rats in each group were treated with T10 total laminectomy to establish an animal model of thoracic spinal cord transection injury. After 24 hours, the olfactory ensheathing cell transplantation group and the combined group were injected with olfactory ensheathing cells near the injured area. Repeated transcranial magnetic stimulation was performed on the left anterior fontanelle of rats in the combined group and repeated transcranial magnetic stimulation group. Results and conclusion: four weeks after transplantation, compared with the other three groups, the rats in the group of repeated transcranial magnetic stimulation combined with olfactory ensheathing cells showed obvious recovery of motor function, increased BBB score of motor function, and obvious recovery of electrophysiological function of sciatic nerve in the group of repeated transcranial magnetic stimulation and olfactory ensheathing cell transplantation. The injured spinal cord was well repaired. The results suggest that repeated transcranial magnetic stimulation combined with olfactory ensheathing cell transplantation can obtain significant therapeutic effect on spinal cord injury in rats. Combined use of both can significantly promote axon regeneration and functional recovery after spinal cord injury.
【作者单位】：大连市骨科医院;
【基金】：大连市科技发展计划支持项目(2013E15SF168)~~
【分类号】：R651.2

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