手术治疗腰椎间盘退变的术后早期复发率与COL9A2基因多态性相关性研究

发布时间：2019-03-04 17:49

【摘要】：目的:目前COL9A2基因突变对腰椎间盘退变术后复发率影响的报道较少,尤其针对国内人群的结果并未明确。本研究旨在探讨COL9A2基因多态性对腰椎间盘退变术后复发率的相关影响。方法:纳入2013年1月至2014年6月在本院经受手术治疗的146名(77名男性,69名女性)腰椎间盘退变患者,将患者分为两组,其中74名患者(39名男性,35名女性,平均年龄49.8岁)在第一次手术后24个月内病情复发,另一组72名患者(38名男性,34名女性,平均年龄49.7岁)在手术治疗后24个月内未出现复发情况。通过术后随访采集,经患者知情同意后,收集外周血,并对其基因扩增产物行限制性片段分析。结果:(1)所有的样本经内切酶BsmF1酶切后产物结果为:复发组与无复发组中,分别有71例、68例患者为未含Trp的类型,分别有3例、4例患者为含Trp杂合子类型。(2)限制性内切酶BceA1酶切结果为:复发组与无复发组中,分别有10例、2例患者为(Arg/Arg)类型,分别有54例、58例患者为(Gln/Gln)类型,分别有10例、12例患者为(Arg/Gln)类型。(3)与无复发组相比,复发组中精氨酸纯合子数量占比更高,有统计学意义(P=0.031)。结论:COL9A2基因多态性与国内人群腰椎间盘退变术后早期复发存在相关性。
[Abstract]:Aim: there are few reports about the effect of COL9A2 gene mutation on the postoperative recurrence rate of lumbar intervertebral disc degeneration, especially in domestic population. The aim of this study was to investigate the correlation between COL9A2 gene polymorphism and postoperative recurrence rate of lumbar intervertebral disc degeneration. Methods: 146 patients (77 males and 69 females) with lumbar disc degeneration underwent surgical treatment in our hospital from January 2013 to June 2014 were divided into two groups: 74 patients (39 males and 35 females). The mean age was 49.8 years old) within 24 months after the first operation. In another group, 72 patients (38 males, 34 females, mean age 49.7 years) did not recur within 24 months after the first operation, and the other 72 patients (38 males and 34 females, mean age 49.7 years) did not recur within 24 months after the first operation. After follow-up, the peripheral blood was collected with the informed consent of the patients, and the amplified products were analyzed by restriction fragment analysis. Results: (1) all the samples were digested by endonuclease BsmF1 and the results were as follows: 71 cases of recurrence group and no recurrence group, 68 cases of Trp-free type, 3 cases of non-recurrence group, 3 cases of non-recurrence group and 3 cases of non-recurrence group, respectively. Trp heterozygote was found in 4 patients. (2) the results of restriction endonuclease BceA1 digestion showed that there were 10 cases in recurrence group and no recurrence group, 2 cases were Arg/Arg type, 54 cases were Gln/Gln type, 58 cases were Gln/Gln type, and 5 cases in non-recurrence group, 2 cases were Arg/Arg type, 58 cases were Gln/Gln type, 2 cases were Arg/Arg type, 58 cases were Gln/Gln type, and 2 cases were Gln/Gln type. (3) compared with the non-recurrent group, the percentage of arginine homozygotes was higher in the recurrent group than that in the non-recurrent group (P < 0. 031). (3) the number of arginine homozygotes in the recurrent group was higher than that in the non-recurrent group (P < 0. 031). Conclusion: there is a correlation between COL9A2 gene polymorphism and early recurrence of lumbar intervertebral disc degeneration in China.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R687.3

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