TSG-6在大鼠静脉桥血管增生中的表达及意义

发布时间：2019-04-09 08:16

【摘要】：目的观察肿瘤坏死因子α刺激基因-6(TSG-6)在大鼠移植静脉壁中的表达,探讨其作用及可能的机制,为临床治疗移植后血管再狭窄提供新方向和理论依据。方法采用袖套法将大鼠自体颈外静脉移植入同侧颈动脉制作血管移植模型,实验分为假手术组、支架组和非支架组。术后12周切除移植静脉,应用苏木精-伊红观察移植静脉内膜、中膜、外膜增生情况,采用计算机图像分析系统测量和计算各膜厚度;应用Western blot检测移植静脉壁内TSG-6、肿瘤坏死因子(TNF)-α和白介素(IL)-1的表达。结果假手术组和支架组的TSG-6、TNF-α和IL-1表达水平、移植静脉内膜增生程度均显著低于非支架组(P0.05)。结论 TSG-6在大鼠静脉桥血管中表达明显升高,并与TNF-α、IL-1炎症因子表达呈正相关性;外支架可能通过抑制移植静脉的炎症反应而限制移植血管壁增生。
[Abstract]:Aim to observe the expression of tumor necrosis factor 伪 stimulating gene-6 (TSG-6) in the transplanted vein wall of rats, and to explore its effect and possible mechanism, so as to provide a new direction and theoretical basis for clinical treatment of vascular restenosis after transplantation. Methods the autogenous external jugular vein was transplanted into the ipsilateral carotid artery by sleeve technique. The model was divided into three groups: sham operation group, stent group and non-stent group. The graft vein was resected 12 weeks after operation. The intima, media and adventitia of the vein were observed by hematoxylin-eosin, and the thickness of each membrane was measured and calculated by computer image analysis system. The expression of TSG-6, tumor necrosis factor-伪 (TNF)-伪) and interleukin-1 (IL)-1) were detected by Western blot. Results the expression of TSG-6,TNF- 伪 and IL-1 and the degree of intimal hyperplasia of vein graft in sham operation group and stent group were significantly lower than those in non stent group (P0.05). Conclusion the expression of TSG-6 is significantly increased in vein bridged vessels of rats, which is positively correlated with the expression of TNF- 伪 and IL-1 inflammatory factors, and external stents may restrict the proliferation of transplanted vessels by inhibiting the inflammatory response of vein grafts.
【作者单位】：安徽医科大学第一附属医院心脏外科;
【基金】：安徽省科技厅重点科研资助项目(编号:1301043025)
【分类号】：R654.2

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