基质金属蛋白酶-9表达下调在异丙酚麻醉诱发大鼠学习记忆障碍中的作用

发布时间：2019-04-21 15:04

【摘要】：目的探讨基质金属蛋白酶-9(MMP-9)表达下调在异丙酚麻醉诱发大鼠学习记忆障碍中的作用。方法 7 d龄SD大鼠随机分为3组(n=18):对照组(NS组)和多次异丙酚麻醉组(RP组)连续7 d分别腹腔注射生理盐水和异丙酚,单次异丙酚麻醉组(SP组)前6天连续腹腔注射生理盐水,d 7注射异丙酚。各组随机选取6只大鼠进行动脉血气和血糖监测,剩余大鼠行Morris水迷宫测试其学习记忆能力,Western bolt法检测MMP-9、BDNF、caspase-3的表达;TUNEL染色法测定海马神经元的凋亡。结果与NS组和SP组相比,RP组大鼠逃逸潜伏期明显延长,探索时间和穿越平台次数明显减少(P0.05),海马MMP-9、m BDNF表达减弱,pro BDNF表达、pro BDNF/m BDNF比值增加(P0.05),神经元凋亡细胞数量明显增多,pro-caspase-3表达减低,cleaved-caspase-3表达增高(P0.05)。而SP组与NS组相比,各结果差异均无显著性(P0.05)。结论多次注射异丙酚导致新生大鼠远期学习记忆能力下降,可能与海马MMP-9表达下调,pro BDNF、m BDNF成熟转换障碍以及海马神经元凋亡发生有关;而单次注射异丙酚对此影响不明显。
[Abstract]:Objective to investigate the down-regulation of matrix metalloproteinase-9 (MMP-9) expression in learning and memory impairment induced by propofol anesthesia in rats. Methods 7-day-old SD rats were randomly divided into 3 groups (n = 18): control group (NS group) and multiple propofol anesthesia group (RP group). Single propofol anesthesia group (SP group) received intraperitoneal injection of normal saline for 6 days and propofol for 7 days. Six rats in each group were randomly selected for arterial blood gas and blood glucose monitoring, the remaining rats were tested with Morris water maze test for learning and memory ability, and the expression of MMP-9,BDNF,caspase-3 was detected by, Western bolt method, and the apoptosis of hippocampal neurons was detected by TUNEL staining. Results compared with NS group and SP group, the escape latency in RP group was significantly prolonged, the exploration time and the times of crossing platform were significantly decreased (P0.05), and the expression of MMP-9,m BDNF in hippocampus decreased, pro BDNF expression. The ratio of pro BDNF/m BDNF increased (P0.05), the number of apoptotic neurons increased significantly, the expression of pro-caspase-3 decreased and the expression of cleaved-caspase-3 increased (P0.05). There was no significant difference between SP group and NS group (P0.05). Conclusion the long-term learning and memory ability of newborn rats induced by multiple injection of propofol may be related to the down-regulation of MMP-9 expression in hippocampus, the disturbance of pro BDNF,m BDNF mature transformation and the occurrence of apoptosis of hippocampal neurons. The effect of single injection of propofol was not obvious.
【作者单位】：重庆医科大学附属第一医院麻醉科;
【基金】：重庆市卫生局医学科研计划(No 2012-1-018) 卫生部国家临床重点专科建设项目[财社(No 2011)170号] 重庆市医学重点学科[渝卫科教(No 2007)2号]
【分类号】：R614

【参考文献】