异氟烷预处理增强大鼠海马内M2型小胶质细胞活化减轻电磁脉冲所致神经损伤

发布时间：2019-06-14 15:17

【摘要】：目的:观察异氟烷预处理是否通过增强大鼠海马内M2型小胶质细胞活化,减轻炎症反应改善电磁脉冲所致神经元损伤。方法:24只SD大鼠随机分为4组,分别为对照组(CON组)、异氟烷预处理组(IP组)和电磁脉冲照射组(EMP组)和异氟烷预处理加电磁脉冲照射组(IP+EMP组)。各组于照射后24 h进行尼氏染色;采用Western Blot和免疫荧光组织化学染色方法检测M2型小胶质细胞标志物Arg-1的表达;并采用ELISA方法测定海马组织中TNF-α和IL-1β的含量。结果:与CON组相比,EMP组海马齿状回颗粒细胞的尼氏体明显减少,而与EMP组相比,IP+EMP组海马齿状回颗粒细胞的尼氏体明显增多增大。Western Blot结果显示:与CON组相比,EMP组海马内Arg-1蛋白的表达明显减少(P0.05),与EMP组相比,IP+EMP组Arg-1蛋白的表达明显增加(P0.05)。免疫荧光染色结果显示:与CON组相比,EMP组海马内Arg-1的表达减弱;而与EMP组相比,IP+EMP组Arg-1的表达明显增强。EMP组海马组织中炎症因子TNF-α和IL-1β的含量较CON组增加(P0.05),IP+EMP组脑组织中炎症因子TNF-α和IL-1β的含量较EMP组减少(P0.05)。结论:异氟烷预处理可通过增加M2型小胶质细胞,减少脑组织中炎症因子含量,减轻EMP所致神经元损伤。
[Abstract]:Aim: to observe whether isohalothane pretreatment can improve neuronal injury induced by electromagnetic pulse by enhancing the activation of M2 microglia in rat hippocampus and reducing inflammation. Methods: 24 SD rats were randomly divided into 4 groups: control group (CON group), isohalothane pretreatment group (IP group), electromagnetic pulse irradiation group (EMP group) and isohalothane pretreatment plus electromagnetic pulse irradiation group (IP EMP group). Nissl staining was performed 24 hours after irradiation, the expression of M2 microglia marker Arg-1 was detected by Western Blot and immunofluorescence histochemical staining, and the contents of TNF- 伪 and IL-1 尾 in hippocampus were measured by ELISA. Results: compared with CON group, the nainite of dentate gyrus granulosa cells in EMP group was significantly lower than that in EMP group, while that in, IP EMP group was significantly higher than that in EMP group. Western Blot results showed that the expression of Arg-1 protein in hippocampus of EMP group was significantly lower than that of CON group (P 0.05), and the expression of Arg-1 protein in, IP EMP group was significantly higher than that of EMP group (P 0.05). The results of immunofluorescence staining showed that the expression of Arg-1 in hippocampus of EMP group was lower than that of CON group, while the expression of Arg-1 in, IP EMP group was significantly higher than that of EMP group. The contents of TNF- 伪 and IL-1 尾 in hippocampal tissue of EMP group were higher than those of CON group (P 0.05). The contents of TNF- 伪 and IL-1 尾 in brain tissue of), IP EMP group were lower than those of EMP group (P 0.05). Conclusion: isofluoroalkane pretreatment can reduce the content of inflammatory factors in brain tissue and reduce neuronal injury induced by EMP by increasing M2 microglia.
【作者单位】：西安市第四医院麻醉科;第四军医大学唐都医院麻醉科;第四军医大学放射医学教研室;
【基金】：国家自然科学基金(3157040065)
【分类号】：R614

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