不同剂量姜黄素腹腔注射对脊髓损伤大鼠脊髓组织结构及后肢运动功能的改善作用

发布时间：2019-06-28 16:09

【摘要】：目的观察不同剂量姜黄素腹腔注射对脊髓损伤(SCI)大鼠脊髓组织结构及运动功能的影响。方法将35只SD大鼠随机分为姜黄素高剂量组、姜黄素中剂量组、姜黄素低剂量组、SCI组和对照组。除对照组外的4组大鼠用HI-0400脊髓打击器制备SCI模型。姜黄素高、中、低剂量组大鼠SCI后30 min腹腔注射200、100、50 mg/kg姜黄素。对照组常规饲养并在相同时间点腹腔注射等量生理盐水。SCI后1、3、7 d取各组大鼠脊髓组织行HE染色光镜下观察脊髓组织形态。SCI后7、14、21、28 d行BBB评分和改良Rivlin斜板实验评价各组大鼠后肢运动功能。结果 SCI组大鼠损伤后1 d可以观察到脊髓局灶性出血,灰质和白质交界线不清晰;很多神经元聚合,胞质内空泡较多,而且还伴随炎症细胞的浸润。损伤后3 d损伤区域出血量有所减少,脂肪液化,细胞核破碎,大量细胞溶解消失,大多胞核萎缩消失,灰质中心明显坏死,灰质内轴索紊乱不规则,胞质内有更多的空泡形成,细胞凋亡明显增多;损伤后7 d大鼠SCI程度有所好转,出血现象基本消失,虽然灰质、白质内仍可见少量液化坏死,空泡,细胞丢失现象有所减轻。姜黄素高、中、低剂量组大鼠脊髓组织形态较SCI组改善,脊髓神经元变性坏死及脊髓组织炎细胞浸润较轻,神经元凋亡的数目较少。SCI后7、14、21、28 d,SCI组大鼠BBB评分和Rivlin斜板实验最大斜板角度明显低于对照组(P均0.05)。随着时间推移,SCI组和姜黄素高、中、低剂量组大鼠SCI后BBB评分和Rivlin斜板实验最大斜板角度逐渐升高(P均0.05)。相同时点BBB评分和Rivlin斜板实验最大斜板角度姜黄素中剂量组小于对照组(P均0.05),姜黄素低、高剂量组小于姜黄素中剂量组(P均0.05),SCI组小于姜黄素低、高剂量组(P均0.05)。结论姜黄素腹腔注射可以促进SCI大鼠脊髓组织结构及运动功能的恢复,100 g/kg姜黄素促进SCI大鼠脊髓运动功能恢复的效果最好。
[Abstract]:Objective to observe the effects of different doses of curcumin on spinal cord tissue structure and motor function in (SCI) rats with spinal cord injury. Methods 35 SD rats were randomly divided into curcumin high dose group, curcumin medium dose group, curcumin low dose group, SCI group and control group. The SCI model was established by HI-0400 spinal cord percussion apparatus in 4 groups except the control group. Rats in high, middle and low dose curcumin groups were intraperitoneally injected with 200, 100, 50 mg/kg curcumin 30 min after SCI. The spinal cord tissue of each group was observed by HE staining on the 7th day after sci. BBB score and modified Rivlin oblique plate test were performed to evaluate the motor function of the hindlimb of the rats in each group on the 7th day after sci. On the 7th, 14th, 21st and 28th day, the motor function of the hindlimb of the rats in each group was evaluated by routine feeding and intraabdominal injection of the same amount of saline at the same time point. Results focal hemorrhage of spinal cord was observed 1 day after injury in SCI group, the boundary between gray matter and white matter was not clear, and many neurons converged, there were more vacuoles in cytoplasm, and the infiltration of inflammatory cells was accompanied by the infiltration of inflammatory cells. On the 3rd day after injury, the amount of bleeding in the injured area decreased, fat liquefaction, nuclear fragmentation, a large number of cells dissolved and disappeared, most of the nucleus atrophy disappeared, the gray matter center obviously died, the axonal disorder in the gray matter was irregular, more vacuoles were formed in the cytoplasm, and the apoptosis was obviously increased. On the 7th day after injury, the degree of SCI was improved and the bleeding phenomenon disappeared basically. Although a small amount of liquefaction necrosis, vacuole and cell loss were found in the gray matter and white matter, the loss of cells was alleviated. Compared with the SCI group, the morphology of spinal cord tissue in the high, middle and low dose groups was improved, the degeneration and necrosis of spinal cord neurons and the infiltration of inflammatory cells in spinal cord tissue were less, and the number of neuronal apoptosis was less. 7 days after sci, 14, 21, 28 days after sci, the BBB score and the maximum oblique plate angle of Rivlin oblique plate test in sci group were significantly lower than those in control group (P 0.05). With the passage of time, the BBB score and the maximum oblique plate angle of SCI group and curcumin group increased gradually after SCI, and the maximum oblique plate angle of Rivlin oblique plate test increased gradually. At the same time, the BBB score and the maximum oblique plate angle of curcumin in the middle dose group were lower than those in the control group (P < 0.05), and the curcumin in the high dose group was lower than that in the curcumin middle dose group (P < 0.05), SCI), and that in the high dose group was lower than that in the high dose group (P < 0.05). Conclusion Intraabdominal injection of curcumin can promote the recovery of spinal cord tissue structure and motor function in SCI rats, and 100 g/kg curcumin has the best effect on the recovery of spinal cord motor function in SCI rats.
【作者单位】：延安大学医学院;
【基金】：国家自然科学基金资助项目(81650022,81641048) 陕西省教育厅科研项目(15JK1813)
【分类号】：R651.2

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