曲尼司特对兔增生性瘢痕组织的抑制作用及其机制研究

发布时间：2019-07-14 19:15

【摘要】：目的:研究曲尼司特对兔增生性瘢痕组织的抑制作用及其机制。方法:取兔建立增生性瘢痕模型,将建模成功的兔随机分为模型对照组(生理盐水)和曲尼司特低、中、高剂量组(0.3、0.5、0.7 mg/kg),每组6只,局部sc相应药物。各组兔分别于注射前1 h和注射后第1、3、5周测量瘢痕厚度,观察注射后第5周瘢痕病理学变化,并检测瘢痕组织中TGF-β_1、α-SMA mRNA和蛋白表达。结果:与注射前1 h比较,除模型对照组外,其余各组兔注射后各时间点瘢痕厚度均减小(P0.05)。注射后第5周,与模型对照组比较,其余各组兔注射后第5周的瘢痕厚度均减小,病理学变化均好转,瘢痕组织中TGF-β_1、α-SMA mRNA和蛋白表达均降低(P0.05),且与曲尼司特剂量呈正相关。结论:曲尼司特能够抑制增生性瘢痕的形成,其作用机制可能与抑制瘢痕组织中TGF-β_1、α-SMA mRNA和蛋白的表达有关。
[Abstract]:Objective: to study the inhibitory effect and mechanism of tranilast on hypertrophic scar tissue in rabbits. Methods: rabbit hypertrophic scar model was established. The rabbits were randomly divided into model control group (saline) and tranilast low, medium and high dose group (0.3, 0.5, 0.7 mg/kg), 6 rabbits in each group, local sc corresponding drugs. The thickness of scar was measured 1 hour before injection and 1, 3 and 5 weeks after injection, the pathological changes of scar were observed at the 5th week after injection, and the expression of TGF- 尾 _ 1, 伪-SMA mRNA and protein in scar tissue were detected. Results: compared with 1 hour before injection, except the model control group, the scar thickness of the other groups decreased at each time point after injection (P 0.05). At the 5th week after injection, compared with the model control group, the scar thickness of the other groups decreased, the pathological changes improved, and the expression of TGF- 尾 _ 1, 伪-SMA mRNA and protein in the scar tissue decreased (P 0.05), which was positively correlated with the dose of tranilast. Conclusion: tranilast can inhibit the formation of hypertrophic scar, and its mechanism may be related to the inhibition of the expression of TGF- 尾 _ 1, 伪-SMA mRNA and protein in scar tissue.
【作者单位】：日照市皮肤病防治所医学美容科;日照市中医医院眼科;日照市人民医院心血管外科;
【分类号】：R622

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