半乳糖凝集素-1联合雷公藤内酯醇对急性脊髓损伤的保护作用及机制

发布时间：2019-07-23 11:54

【摘要】：目的探讨半乳糖凝集素-1联合雷公藤内酯醇对急性脊髓损伤的保护作用及机制。方法 60只无特殊病原菌的雄性SD大鼠,随机均分为A、B、C、D、E组。通过Allen's法打击进行脊髓造模。A组腹腔注射半乳糖凝集素-1 4 mg/kg联合雷公藤内酯醇0.4 mg/kg,1次/d,持续1周;B组腹腔注射半乳糖凝集素-1 4 mg/kg,1次/d,持续1周;C组腹腔注射雷公藤内酯醇0.4 mg/kg,1次/d,持续1周;D组腹腔注射甲基强的松龙30 mg/kg,12 h后再重复1次,以后1次/d,重复3 d;E组腹腔注射生理盐水3 mg/kg,1次/d,持续1周。分别于术后1、3、7、14 d对各组大鼠进行行为学评价(BBB评分+斜板倾斜度);于术后14 d行病变脊髓组织苏木精-伊红染色法及免疫组化染色检查,同时用ELISA法测定IL-10及TNF-α表达水平。结果术后7、14 d,A组BBB评分及斜板倾斜度较其余组高(P均0.05);其中B、C、D组间比较,P均0.05。术后14 d的HE染色结果显示A组的脊髓组织神经元数量多于其余组(P均0.05)。免疫组化染色结果提示,A组脊髓组织的GAP-43阳性表达水平较其余组高(P均0.05)。术后7 d及14 d,与其余组比较,A组TNF-α表达水平低,IL-10水平高(P均0.05),而B、C、D组间比较,P均0.05。结论半乳糖凝集素-1联合雷公藤内酯醇可减轻急性脊髓损伤的进一步损害,其作用机制可能与上调IL-10及下调TNF-α的表达水平有关。
[Abstract]:Objective To study the protective effect and mechanism of galectin-1 combined with triptolide on acute spinal cord injury. Methods 60 male SD rats with no special pathogen were randomly divided into A, B, C, D and E groups. The spinal cord was model by Allen's method. A group of intraperitoneal injection of galectin-1 (4 mg/ kg) combined with triptolide (0.4 mg/ kg,1 time/ day for 1 week) was performed. In group D, methylprednisolone (30 mg/ kg) was injected into the abdominal cavity (30 mg/ kg), and then repeated for 1 time after 12 h, and then repeated for 3 days. The intraperitoneal injection of saline in group E was 3 mg/ kg and 1 time/ day for 1 week. Behavior evaluation (BBB score + inclined plate inclination) was performed on each group of rats at 1,3,7, and 14 days after operation, and the expression levels of IL-10 and TNF-1 were determined by ELISA, and the expression levels of IL-10 and TNF-1 were determined by ELISA. Results The BBB score and the inclination of the inclined plate were higher in group A than in other groups (P <0.05). The results of HE staining showed that the number of spinal cord in group A was more than that of the rest (P 0.05). The results showed that the positive expression level of GAP-43 in group A was higher than that in the other group (P <0.05). The levels of TNF-1 and IL-10 in group A were lower in group A and higher in group B, C and D after 7 d and 14 days after operation (P <0.05), and P was 0.05 in group B, C and D. Conclusion Galectin-1 and triptolide can reduce the further damage of acute spinal cord injury, and its mechanism of action may be related to up-regulation of IL-10 and down-regulation of the expression level of TNF-1.
【作者单位】：三峡大学第一临床医学院·宜昌市中心人民医院;
【分类号】：R651.2

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