前列腺素E1后处理对大鼠移植肝缺血再灌注损伤的保护作用

发布时间：2019-08-01 11:22

【摘要】：目的探讨前列腺素E1(PGE1)后处理对大鼠移植肝缺血再灌注损伤(IRI)的保护作用及其机制。方法建立SD大鼠肝移植模型,随机分为3组:IRI组、PGE1后处理组(PGE1组)及假手术组(S组)。检测各组血清谷丙转氨酶(ALT)、谷草转氨酶(AST)、超氧化物岐化酶(SOD)及丙二醛(MDA)的含量;采用逆转录聚合酶链式反应(RT-PCR)方法检测移植肝组织内肿瘤坏死因子(TNF-α)信使核糖核酸(mRNA)的表达水平;苏木精-伊红染色(HE)观察移植肝病理形态学变化。结果供肝再灌注后2及6 h,PGE1组的大鼠血清ALT、AST和MDA水平及TNF-αmRNA的表达低于IRI组(P0.05),血清SOD水平高于IRI组(P0.05),光镜下PGE1组肝组织损伤较IRI组轻。结论 PGE1后处理可通过提高肝组织的抗氧化能力,降低TNF-α来减轻大鼠移植肝IRI。
[Abstract]:Objective to investigate the protective effect and mechanism of prostaglandin E1 (PGE1) posttreatment on (IRI) after liver ischemia-reperfusion injury in rats. Methods SD rat model of liver transplantation was established and randomly divided into three groups: IRI group, PGE1 post-treatment group (PGE1 group) and pseudo-operation group (S group). The contents of serum glutamic pyruvic transaminase (ALT), glutamic oxaloacetic transaminase (AST), (SOD) and malondialdehyde (MDA) were detected, the expression of tumor necrosis factor 伪 (TNF- 伪) messenger ribonucleic acid (mRNA) was detected by reverse transcriptional polymerase chain reaction (RT-PCR), and the pathomorphological changes of the transplanted liver were observed by hematoxylin and eosin staining (HE). Results the levels of serum ALT,AST and MDA and the expression of TNF- 伪 mRNA in PGE 1 group were lower than those in IRI group (P 0.05), and the serum SOD level was higher than that in IRI group (P 0.05). The liver tissue injury in PGE1 group was less than that in IRI group under light microscope. Conclusion PGE1 post-treatment can reduce IRI. after liver transplantation by improving the antioxidant capacity of liver tissue and decreasing TNF- 伪.
【作者单位】：郑州大学人民医院(河南省人民医院)肝胆胰腺外科;中南大学湘雅移植医学研究院;
【基金】：河南省科技攻关计划资助项目(No:162102310303)
【分类号】：R657.3

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