TGF-β1/miR-411/SPRY4和MAPK信号通路调控横纹肌肉瘤增殖与分化的机制研究
发布时间：2021-12-29 00:02
目的:探讨TGF-β1抑制的microRNAs及其靶基因蛋白通过MAPK信号通路调节人类横纹肌肉瘤（RMS）肌分化及其分子机制,为RMS分化治疗提供新的治疗靶点。方法:（1）采用Real-time PCR技术检测TGF-β1抑制的microRNAs在不同RMS细胞株和组织样本中的表达,筛选出差异最明显、最广泛的microRNA作为研究对象。（2）利用MTT、3H胸腺嘧啶核苷掺入法检测microRNA对RMS细胞株增殖的影响。（3）构建裸鼠成瘤性模型,观察microRNA对活体肿瘤生长的影响;采用免疫组织化学染色检测瘤体ki-67蛋白表达情况。（4）运用生物信息学技术及网络资源预测miR-411-5p的靶基因（5）运用Real-time PCR技术、双荧光素酶报告系统及蛋白印迹法验证靶基因。（6）构建Elk-1-Luc、c-Jun-Luc荧光报告质粒,SPRY4-siRNA或siRNA-ctrl转染PKC?表达质粒处理的RMS细胞株,检测Elk-1-Luc、c-Jun-Luc荧光信号;PKC?表达质粒和SPRY4-siRNA/siRNA-ctrl共转染RMS细胞株,检测P-p38蛋白的变...
【文章来源】：苏州大学江苏省 211工程院校
【文章页数】：83 页
【学位级别】：硕士
【部分图文】：
验证TGF-β1基因敲低效果
TGF-β1/miR-411-5p/SPRY4和 MAPK信号通路参与横纹肌肉瘤分化调表达明显增加 (图 2 )。miR-411-5p 和 miR-411-3p 在 RMS、更普遍，因此选择 miR-411-5p 在 RMS 组织中进一步验
RT-PCR检测miR-411-5p六对低/高TGF-β1表达的ARMS和八对ERMS组织标本
【参考文献】：
期刊论文
[1]Antisense Oligonucleotide Targeting TGF-β1 Abrogates Tumorigenicity of Rhabdomyosarcoma in vivo[J]. Shouli Wang Lingling Guo Liang Dong Shigang Li Haizhen Deng Department of Pathology,Medical School of Soochow University,Suzhou 215123,Jiangsu Province,China. Huihua Yao Department of Surgery,the Second Hospital of Soochow University,Suzhou 215123,Jiangsu Province,China. Maomin Sun Department of Anatomy,Medical School of Soochow University,Suzhou 215123,Jiangsu Province.China.. Chinese Journal of Clinical Oncology. 2008(04)
[2]Inhibition of Differentiation by Transforming Growth Factor β1 in Rhabdomyosarcoma Cells[J]. Shouli Wang1 Huihua Yao2 Zhenghong Qin3 Shigang Li1 Yizhong Feng1 Xiuzhen Wang1 Min Deng1 Lingling Guo1 Lifeng Zhang1 1 Molecular Pathology Laboratory,Medical School of Soochow University,Suzhou 215123,China. 2 Department of Surgery,the Second Affiliated Hospital of Soochow University,Suzhou 215123,China. 3 Pharmacology Laboratory,Medical School of Soochow University,Suzhou 215123,China.. Chinese Journal of Clinical Oncology. 2007(05)
[3]Smad4 and ERK2 stimulated by transforming growth factor betal in rhabdomyosarcoma[J]. GUO Hua ZHANG Hong-ying WANG Shou-li YE Lü YANG Guang-hua BU Hong Department of Pathology,West China Hospital,Sichuan University,Chengdu 610041,China (Zhang HY,Wang SL,Ye L,Yang GH and Bu H)Department of Pathology,Peking University First Hospital,Beijing 100034,China (Guo H)Department of Pathology,Medical School,Soochow University,Suzhou 215007,China (Wang SL). Chinese Medical Journal. 2007(06)
本文编号：3554990
【文章来源】：苏州大学江苏省 211工程院校
【文章页数】：83 页
【学位级别】：硕士
【部分图文】：
验证TGF-β1基因敲低效果
TGF-β1/miR-411-5p/SPRY4和 MAPK信号通路参与横纹肌肉瘤分化调表达明显增加 (图 2 )。miR-411-5p 和 miR-411-3p 在 RMS、更普遍，因此选择 miR-411-5p 在 RMS 组织中进一步验
RT-PCR检测miR-411-5p六对低/高TGF-β1表达的ARMS和八对ERMS组织标本
【参考文献】：
期刊论文
[1]Antisense Oligonucleotide Targeting TGF-β1 Abrogates Tumorigenicity of Rhabdomyosarcoma in vivo[J]. Shouli Wang Lingling Guo Liang Dong Shigang Li Haizhen Deng Department of Pathology,Medical School of Soochow University,Suzhou 215123,Jiangsu Province,China. Huihua Yao Department of Surgery,the Second Hospital of Soochow University,Suzhou 215123,Jiangsu Province,China. Maomin Sun Department of Anatomy,Medical School of Soochow University,Suzhou 215123,Jiangsu Province.China.. Chinese Journal of Clinical Oncology. 2008(04)
[2]Inhibition of Differentiation by Transforming Growth Factor β1 in Rhabdomyosarcoma Cells[J]. Shouli Wang1 Huihua Yao2 Zhenghong Qin3 Shigang Li1 Yizhong Feng1 Xiuzhen Wang1 Min Deng1 Lingling Guo1 Lifeng Zhang1 1 Molecular Pathology Laboratory,Medical School of Soochow University,Suzhou 215123,China. 2 Department of Surgery,the Second Affiliated Hospital of Soochow University,Suzhou 215123,China. 3 Pharmacology Laboratory,Medical School of Soochow University,Suzhou 215123,China.. Chinese Journal of Clinical Oncology. 2007(05)
[3]Smad4 and ERK2 stimulated by transforming growth factor betal in rhabdomyosarcoma[J]. GUO Hua ZHANG Hong-ying WANG Shou-li YE Lü YANG Guang-hua BU Hong Department of Pathology,West China Hospital,Sichuan University,Chengdu 610041,China (Zhang HY,Wang SL,Ye L,Yang GH and Bu H)Department of Pathology,Peking University First Hospital,Beijing 100034,China (Guo H)Department of Pathology,Medical School,Soochow University,Suzhou 215007,China (Wang SL). Chinese Medical Journal. 2007(06)
本文编号：3554990
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