二氢丹参酮Ⅰ（DHI）对骨肉瘤影响及机制研究

发布时间：2021-12-31 12:21

　　目的:探讨二氢丹参酮Ⅰ（Dihydrotanshinone I,DHI）对骨肉瘤细胞的抑制作用及其机制。方法:采用结晶紫染色法检测浓度不同的DHI对人骨肉瘤细胞（MG-63、143B、Saos2、U2OS）增殖能力的影响;用MTT法进一步验证DHI对人骨肉瘤细胞（MG-63、143B）增殖能力的作用。通过克隆形成实验、Transwell迁移侵袭实验、细胞划痕实验、Hoechst 33258染色以及应用流式细胞术等实验方法检测浓度不同浓度的DHI对人骨肉瘤细胞（MG-63、143B）克隆形成能力、迁移能力、侵袭能力及凋亡能力的影响。随后用浓度不同的DHI（Control、0、2、4、6μM）处理人骨肉瘤143B细胞,通过蛋白印迹法（Western Blot）检测用DHI处理的人骨肉瘤143B细胞和未用DHI处理的人骨肉瘤143B细胞中增殖细胞核抗原（PCNA）、Snail、MMP-2、MMP-7、MMP-9、N-Cadherin、Bcl-2、PARP、Cleaved–PARP、Caspase-3、Cleaved Caspase-3、LRP6、β-Catenin、Cyclin D1、c-M... 

【文章来源】：重庆医科大学重庆市

【文章页数】：46 页

【学位级别】：硕士

【部分图文】：

DHI抑制骨肉瘤细胞增殖Figure1DHIinhibitedOScellproliferationinvitro(A-F)DHIsuppressedOScellproliferation,asmeasuredbycrystalvioletstaining.(G-H)DHIsuppressedOScellproliferation,asmeasuredbytheMTTassay.(I-L)ColonyformationassaysshowedasignificantlylowercolonyformingrateinbothcelllinesfollowingDHItreatment.(M-N)

DHI抑制骨肉瘤细胞迁移和侵袭Figure2DHIinhibitedOScellsmigrationandinvasioninvitro(A–D)WoundhealingassaysshowedsignificantlydecreasedmigratoryabilitiesinbothcelllinesfollowingDHItreatment.(E–H)TranswellassaysshowedsignificantlydecreasedmigrationabilitiesinbothcelllinesfollowingDHItreatment.(I–L)TranswellassaysshowedsignificantlydecreasedinvasiveabilitiesinbothcelllinesfollowingDHItreatment.(M-N)Westernblotanalysisshowi

重庆医科大学硕士研究生学位论文(A-D) Flow cytometry was used to explore whether DHI promoted OS cell apoptosis. (E-H)Hoechst staining showed that DHI significantly promoted apoptosis of two cell lines. (I-J) Westernblot analysis showing DHI downregulated Bcl-2, and upregulated PARP, Cleaved PARP, Caspase-3,Cleaved Caspase-3. (K) The Δψm (red/green) alteration in 143B cells was determined byfluorescence microscopy after staining with JC-1. μM: The different concentrations of DHI; Control:Osteosarcoma 143B cells were treated with DMSO to exclude the effect of DMSO on the test.△△P＜0.01, vs the control group; **P <0.01, vs 0μmol/L DHI group. (n=3)tu

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期刊论文
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