MMP-2和TIMP-2在激光诱导形觉剥夺的高度近视豚鼠脉络膜新生血管中的表达变化

发布时间：2018-08-18 07:37

【摘要】：目的构建高度近视豚鼠脉络膜血管新生(choroidal neovascularization,CNV)模型,探讨基质金属蛋白酶(matrix metalloproteinase-2,MMP-2)和基质金属蛋白酶抑制剂(tissue inhibitor-2 of metalloproteinase,TIMP-2)在高度近视CNV形成中的作用。方法 72只2周龄三色豚鼠随机分为实验组(n=36)和对照组(n=36),实验组右眼行6周形觉剥夺诱导高度近视。两组各选取30只豚鼠右眼行532 nm激光光凝视网膜诱导CNV。于激光前和光凝后7、14、21、28、35天进行免疫组化观察光凝区域MMP-2和TIMP-2的表达,real-time PCR检测视网膜色素上皮层(retinal pigment epithelium,RPE)-脉络膜-巩膜复合物中的MMP-2和TIMP-2mRNA表达对MMP-2和TIMP-2免疫组化阳性表达的积分光密度值(integral optical desnsity,IDO)及mRNA相对表达水平进行统计学分析。结果激光光凝后实验组和对照组MMP-2和TIMP-2表达均明显上调,MMP-2于光凝后21天、TIMP-2于光凝后28天时表达高峰。激光前及光凝后各观察时间点,实验组MMP-2阳性表达和mRNA相对表达量均明显高于对照组,差异有统计学意义(P0.05);而激光前实验组TIMP-2表达较对照组明显减少(P0.05),光凝后各时间点两组TIMP-2表达差异无统计学意义。结论MMP-2和TIMP-2与高度近视CNV形成密切相关,MMP-2与TIMP-2表达平衡紊乱可能参与高度近视CNV的发生发展。
[Abstract]:Objective to study the role of matrix metalloproteinase-2 (MMP-2) and tissue inhibitor-2 of metalloproteinase (TIMP-2) in the formation of high myopia (CNV) in guinea pigs with high myopia by establishing a choroidal neovascularization model. Methods 72 two-week-old tricolor guinea pigs were randomly divided into two groups: the experimental group (n = 36) and the control group (n = 36). The right eye of the experimental group was induced by 6 weeks of form deprivation to induce high myopia. 30 guinea pigs in each group were treated with 532 nm laser photocoagulation to induce CNV. Immunohistochemical observation of MMP-2 and TIMP-2 expression in the photocoagulation region before and after photocoagulation: detection of MMP-2 and TIMP-2mRNA expression in (retinal pigment epithelium of retinal pigment epithelium layer by real-time PCR and MMP-2 and TIMP-2mRNA expression in chorio-scleral complexes positive for MMP-2 and TIMP-2 The integrated optical density (integral optical) and the relative expression level of mRNA were analyzed statistically. Results after laser photocoagulation, the expression of MMP-2 and TIMP-2 in the experimental group and the control group were significantly up-regulated, and the expression of TIMP-2 was significantly increased on the 21st day after photocoagulation, and the peak expression of TIMP-2 was observed on the 28th day after photocoagulation. The positive expression of MMP-2 and the relative expression of mRNA in the experimental group were significantly higher than those in the control group before and after photocoagulation. The difference was statistically significant (P0.05), while the expression of TIMP-2 in the experimental group was significantly lower than that in the control group (P0.05). There was no significant difference in the expression of TIMP-2 between the two groups at each time point after photocoagulation. Conclusion MMP-2 and TIMP-2 are closely related to the formation of CNV in high myopia. The imbalance of MMP-2 and TIMP-2 expression may be involved in the occurrence and development of CNV in high myopia.
【作者单位】：上海市第十人民医院眼科;上海市光华中西医结合医院眼科;
【基金】：上海市科委基础研究重点项目(11JC1401202)~~
【分类号】：R778.11

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